BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 17912

Title: The third SH3 domain of R85FL   PubMed: 23892081

Deposition date: 2011-09-02 Original release date: 2012-09-14

Authors: Jiang, Ya-Jun; Hu, Hongyu

Citation: Jiang, Ya-Jun; Zhou, Chen-Jie; Zhou, Zi-Ren; Wu, Meng; Hu, Hong-Yu. "Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7."  FEBS Lett. 587, 2905-2911 (2013).

Assembly members:
third SH3 domain of R85FL, polymer, 65 residues, 7581.400 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22b

Entity Sequences (FASTA):
third SH3 domain of R85FL: QTSQDLFSYQALYSYIPQND DELELRDGDIVDVMEKCDDG WFVGTSRRTKQFGTFPGNYV KPLYL

Data sets:
Data typeCount
13C chemical shifts229
15N chemical shifts66
1H chemical shifts396

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1third SH3 domain of R85FL1

Entities:

Entity 1, third SH3 domain of R85FL 65 residues - 7581.400 Da.

1   GLNTHRSERGLNASPLEUPHESERTYRGLN
2   ALALEUTYRSERTYRILEPROGLNASNASP
3   ASPGLULEUGLULEUARGASPGLYASPILE
4   VALASPVALMETGLULYSCYSASPASPGLY
5   TRPPHEVALGLYTHRSERARGARGTHRLYS
6   GLNPHEGLYTHRPHEPROGLYASNTYRVAL
7   LYSPROLEUTYRLEU

Samples:

sample_1: sodium phosphate 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.12 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HNHAsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 17913
PDB
DBJ BAA92534 BAG58006 BAG62137 BAG65026 BAG72789
EMBL CAB55947 CAD34588 CAE45892 CAH89601 CAH90184
GB AAD27647 AAF22175 AAI52464 AAI60134 AAK37563
REF NP_001030126 NP_001030127 NP_001030128 NP_001030129 NP_001068637
SP Q9BX66
AlphaFold Q9BX66

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts