BMRB Entry 17926

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PDB ID: 2lj9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17926
MolProbity Validation Chart

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Title:
PARTIAL 3D STRUCTURE OF THE C-TERMINAL PART OF THE FREE ARAB THALIANA CP12-2 IN ITS OXIDIZED FORM
Deposition date:
2011-09-09
Original release date:
2012-04-23
Authors:
Trivelli, X.; Sparla, F.; Marri, L.; Trost, P.
Citation:

Citation: Fermani, Simona; Trivelli, Xavier; Sparla, Francesca; Thumiger, Anton; Calvaresi, Matteo; Marri, Lucia; Falini, Giuseppe; Zerbetto, Francesco; Trost, Paolo. "Conformational selection and folding-upon-binding of the intrinsically disordered protein CP12 regulate photosynthetic enzymes assembly"  J. Biol. Chem. 287, 21372-21383 (2012).
PubMed: 22514274

Assembly members:

Assembly members:
CP12_PROTEIN, polymer, 99 residues, 2637.736 Da.

Natural source:

Natural source:   Common Name: Thale cress   Taxonomy ID: 3702   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CP12_PROTEIN: MGSSHHHHHHSSGLVPRGSH MAAPEGGISDVVEKSIKEAQ ETCAGDPVSGECVAAWDEVE ELSAAASHARDKKKADGSDP LEEYCKDNPETNECRTYDN

Data sets:
Data typeCount
13C chemical shifts187
15N chemical shifts53
1H chemical shifts278
heteronuclear NOE values19

Additional metadata:

  • Assembly
  • Samples and Experiments
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  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CP12 PROTEIN-LIKE PROTEIN1

Entities:

Entity 1, CP12 PROTEIN-LIKE PROTEIN 99 residues - 2637.736 Da.

Residues 1-21 (MGS...SHM) represent a non-native affinity tag. Numeration of the protein starts at AAPEGG...

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METALAALAPROGLUGLYGLYILESERASP
4   VALVALGLULYSSERILELYSGLUALAGLN
5   GLUTHRCYSALAGLYASPPROVALSERGLY
6   GLUCYSVALALAALATRPASPGLUVALGLU
7   GLULEUSERALAALAALASERHISALAARG
8   ASPLYSLYSLYSALAASPGLYSERASPPRO
9   LEUGLUGLUTYRCYSLYSASPASNPROGLU
10   THRASNGLUCYSARGTHRTYRASPASN

Related Database Links:

PDB 2LJ9 3QV1 3RVD
EMBL CAB82955
GB AAM20142 AAM45071 AAM63795 AEE80349 EFH52933
REF NP_191800 XP_002876674
SP Q9LZP9
AlphaFold Q9LZP9

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