BMRB Entry 17937
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17937
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Sun, Shangjin; Siglin, Amanda; Williams, John; Polenova, Tatyana. "Solid-State and Solution NMR Studies of the CAP-Gly Domain of Mammalian Dynactin and Its Interaction with Microtubules" J. Am. Chem. Soc. 131, 10113-10126 (2009).
PubMed: 19580321
Assembly members:
CAP-Gly, polymer, 88 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b-His6-SMT3
Entity Sequences (FASTA):
CAP-Gly: TEASARPLRVGSRVEVIGKG
HRGTVAYVGATLFATGKWVG
VILDEAKGKNDGTVQGRKYF
TCDEGHGIFVRQSQIQVFED
GADTTSPE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 327 |
| 15N chemical shifts | 85 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | CAP-Gly | 1 |
Entities:
Entity 1, CAP-Gly 88 residues - Formula weight is not available
| 1 | THR | GLU | ALA | SER | ALA | ARG | PRO | LEU | ARG | VAL | ||||
| 2 | GLY | SER | ARG | VAL | GLU | VAL | ILE | GLY | LYS | GLY | ||||
| 3 | HIS | ARG | GLY | THR | VAL | ALA | TYR | VAL | GLY | ALA | ||||
| 4 | THR | LEU | PHE | ALA | THR | GLY | LYS | TRP | VAL | GLY | ||||
| 5 | VAL | ILE | LEU | ASP | GLU | ALA | LYS | GLY | LYS | ASN | ||||
| 6 | ASP | GLY | THR | VAL | GLN | GLY | ARG | LYS | TYR | PHE | ||||
| 7 | THR | CYS | ASP | GLU | GLY | HIS | GLY | ILE | PHE | VAL | ||||
| 8 | ARG | GLN | SER | GLN | ILE | GLN | VAL | PHE | GLU | ASP | ||||
| 9 | GLY | ALA | ASP | THR | THR | SER | PRO | GLU |
Samples:
sample_1: CAP-Gly, [U-100% 13C; U-100% 15N], 14 ± 0.5 mg
sample_conditions_1: ionic strength: 30 mM; pH: 6.0; pressure: 1 atm; temperature: 256.3 K
sample_conditions_2: ionic strength: 30 mM; pH: 6.0; pressure: 1 atm; temperature: 258 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DARR | sample_1 | solid | sample_conditions_2 |
| NCA | sample_1 | solid | sample_conditions_1 |
| NCO | sample_1 | solid | sample_conditions_1 |
| NCACB | sample_1 | solid | sample_conditions_1 |
| NCACX | sample_1 | solid | sample_conditions_1 |
| CTUC-COCA | sample_1 | solid | sample_conditions_1 |
| CTUC-CACB | sample_1 | solid | sample_conditions_1 |
| CTUC-NCOCA | sample_1 | solid | sample_conditions_1 |
| NCOCX | sample_1 | solid | sample_conditions_1 |
| DARR | sample_1 | solid | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment
Rowland NMR Toolkit, (RNMRTK)-Hoch, Stern, Li, Mobli, Maciejewski, Gryk - processing
NMR spectrometers:
- Varian InfinityPlus 600 MHz
- Varian INOVA 900 MHz
Related Database Links:
| BMRB | 17938 19025 19031 25005 |
| PDB | |
| DBJ | BAE34241 BAE37079 BAE42418 BAE42912 BAE87998 |
| EMBL | CAA44091 CAA67333 CAE45882 CAH10572 CAH10575 |
| GB | AAB57773 AAD03694 AAD55811 AAH66061 AAI42510 |
| REF | NP_001092404 NP_001127253 NP_001128512 NP_001177765 NP_001177766 |
| SP | O08788 P28023 Q14203 |
| TPG | DAA24857 |
| AlphaFold | O08788 P28023 Q14203 |