BMRB Entry 18024

Name: 13C and 15N chemical shift assignments of cAMP bound Cyclic Nucleotide-Binding Domain from a bacterial Cyclic Nucleotide-Gated channel
Published: 2012-02-17

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18024

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

13C and 15N chemical shift assignments of cAMP bound Cyclic Nucleotide-Binding Domain from a bacterial Cyclic Nucleotide-Gated channel

Deposition date:

2011-10-26

Original release date:

2012-02-17

Authors:

Cukkemane, Abhishek

Citation:

Citation: Cukkemane, Abhishek; Nand, Deepak; Gradmann, Sabine; Weingarth, Markus; Kaupp, U. Benjamin; Baldus, Marc. "Solid-state NMR [13C,15N] resonance assignments of the nucleotide-binding domain of a bacterial cyclic nucleotide-gated channel." Biomol. NMR Assignments 6, 225-229 (2012).
PubMed: 22302441

Assembly members:

Assembly members:
Cyclic nucleotide binding domain, subunit 1, polymer, 142 residues, Formula weight is not available
CMP, non-polymer, 329.206 Da.

Natural source:

Natural source: Common Name: Mesorhizobium loti Taxonomy ID: 381 Superkingdom: Bacteria Kingdom: not available Genus/species: Mesorhizobium loti

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cyclic nucleotide binding domain, subunit 1: GSQEVRRGDFVRNWQLVAAV PLFQKLGPAVLVEIVRALRA RTVPAGAVICRIGEPGDRMF FVVEGSVSVATPNPVELGPG AFFGEMALISGEPRSATVSA ATTVSLLSLHSADFQMLCSS SPEIAEIFRKTALERRGAAA SA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	434
15N chemical shifts	118

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cyclic nucleotide binding domain, subunit 1	1
2	cyclic adenosine monophosphate	2

Entities:

Entity 1, Cyclic nucleotide binding domain, subunit 1 142 residues - Formula weight is not available

1

GLY

SER

GLN

GLU

VAL

ARG

GLY

ASP

PHE

2

VAL

ARG

ASN

TRP

GLN

LEU

VAL

ALA

VAL

3

PRO

LEU

PHE

GLN

LYS

LEU

GLY

PRO

ALA

VAL

4

LEU

VAL

GLU

ILE

VAL

ARG

ALA

LEU

ARG

ALA

5

ARG

THR

VAL

PRO

ALA

GLY

ALA

VAL

ILE

CYS

6

ARG

ILE

GLY

GLU

PRO

GLY

ASP

ARG

MET

PHE

7

PHE

VAL

GLU

GLY

SER

VAL

SER

VAL

ALA

8

THR

PRO

ASN

PRO

VAL

GLU

LEU

GLY

PRO

GLY

9

ALA

PHE

GLY

GLU

MET

ALA

LEU

ILE

SER

10

GLY

GLU

PRO

ARG

SER

ALA

THR

VAL

SER

ALA

11

ALA

THR

VAL

SER

LEU

SER

LEU

HIS

12

SER

ALA

ASP

PHE

GLN

MET

LEU

CYS

SER

13

SER

PRO

GLU

ILE

ALA

GLU

ILE

PHE

ARG

LYS

14

THR

ALA

LEU

GLU

ARG

GLY

ALA

15

SER

ALA

Entity 2, cyclic adenosine monophosphate - C10 H12 N5 O6 P - 329.206 Da.

1

CMP

Samples:

sample_1: Cylic Nucleotide Binding Domain, [U-100% 13C; U-100% 15N], 10 mg; H2O 95%; D2O 5%; Tris 10 mM

sample_conditions_1: ionic strength: 10 mM; pH: 8; pressure: 1 atm; temperature: 263 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D CC	sample_1	solid	sample_conditions_1
3D NCOCX	sample_1	solid	sample_conditions_1
2D NCACX	sample_1	solid	sample_conditions_1
3D CCC	sample_1	solid	sample_conditions_1

Software:

SPARKY, Bruker Biospin, Cornilescu, Delaglio and Bax, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

Bruker Avance 700 MHz