BMRB Entry 18046

Assembly:

Entities:

Entity 1, D6 107 residues - 12427.814 Da.

Corresponds to 107 residue polypeptide from villin [gallus gallus] starting at position 619 and ending at 725

Samples:

sample_1: H2O 50 M; D2O, [U-2H], 5 M; DTT, [U-2H], 10 mM; sodium azide 0.01%; Calcium Chloride 5 mM; PIPES, [U-2H], 20 mM; D6, [U-99% 13C; U-99% 15N], 1 mM

sample_2: H2O 50 M; D2O, [U-2H], 5 M; DTT, [U-2H], 10 mM; sodium azide 0.01%; Calcium Chloride 5 mM; PIPES, [U-2H], 20 mM; D6 1 mM

sample_3: D2O, [U-2H], 55 M; DTT, [U-2H], 10 mM; sodium azide 0.01%; Calcium Chloride 5 mM; PIPES, [U-2H], 20 mM; D6 1 mM

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 273 K

sample_conditions_2: pH: 7; pressure: 1 atm; temperature: 273 K

sample_conditions_3: pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMRView v5.2.2, Johnson, One Moon Scientific - peak picking

NMRPipe v5.5, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

PINE, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

PREDITOR v1.0, Berjanskii MV, Neal S, Wishart DS - dihedral prediction, geometry optimization, structure solution

NMR spectrometers:

• Varian INOVA 720 MHz
• Varian INOVA 500 MHz