BMRB Entry 18082

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PDB ID: 2llo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18082
MolProbity Validation Chart

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Title:
SOLUTION NMR-DERIVED STRUCTURE OF CALMODULIN N-LOBE BOUND WITH ER alpha PEPTIDE
Deposition date:
2011-11-15
Original release date:
2012-01-30
Authors:
Zhang, Yonghong
Citation:

Citation: Zhang, Yonghong; Li, Zhigang; Sacks, David; Ames, James. "Structural basis for Ca2+-induced activation and dimerization of estrogen receptor by calmodulin."  J. Biol. Chem. 287, 9336-9344 (2012).
PubMed: 22275375

Assembly members:

Assembly members:
Calmodulin N-Lobe, polymer, 80 residues, 8914.874 Da.
ER alpha peptide, polymer, 19 residues, 2202.663 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: African clawed frog   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Calmodulin N-Lobe: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD
ER alpha peptide: RAANLWPSPLMIKRSKKNS

Data sets:
Data typeCount
13C chemical shifts157
15N chemical shifts78
1H chemical shifts295

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Calmodulin N-Lobe1
2ER alpha peptide2
3CA2_13
4CA2_23

Entities:

Entity 1, Calmodulin N-Lobe 80 residues - 8914.874 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP

Entity 2, ER alpha peptide 19 residues - 2202.663 Da.

1   ARGALAALAASNLEUTRPPROSERPROLEU
2   METILELYSARGSERLYSLYSASNSER

Entity 3, CA2_1 - Ca - 40.078 Da.

1   CA

Related Database Links:

BMRB 18302 4174
PDB 1AK8 1F70 1J7O 1SW8 2KUG 2LLO 2LQC 2PQ3 3B32 3IFK 3UCT 3UCW 3UCY 4Q57
EMBL CAA29381 CAB65357 CCD82979 CUA69889
GB AAH53790 ABY16748 ABY16749 ABY16752 ACH46202
REF XP_001869424 XP_001995129 XP_002772218 XP_006032607 XP_006137175
AlphaFold Q9UIS7

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