BMRB Entry 18171

Name: Solution structure of atTic-hip/hop domain (Residue 310-371)
Published: 2012-09-14

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PDB ID: 2lnm
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18171
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of atTic-hip/hop domain (Residue 310-371)

Deposition date:

2012-01-01

Original release date:

2012-09-14

Authors:

Chen, Chinpan; Kao, Yi-Fen

Citation:

Citation: Kao, Yi-Fen; Lou, Yuan-Chao; Yeh, Yi-Hung; Hsiao, Chwan-Deng; Chen, Chinpan. "Solution structure of the C-terminal NP-repeat domain of Tic40, a co-chaperone during protein import into chloroplasts." J. Biochem. 152, 443-451 (2012).
PubMed: 22888115

Assembly members:

Assembly members:
entity, polymer, 62 residues, 7056.178 Da.

Natural source:

Natural source: Common Name: Thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: PEEVISKIMENPDVAMAFQN PRVQAALMECSENPMNIMKY QNDKEVMDVFNKISQLFPGM TG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	262
15N chemical shifts	67
1H chemical shifts	425

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	atTic-hip/hop domain (Residue 310-371)	1

Entities:

Entity 1, atTic-hip/hop domain (Residue 310-371) 62 residues - 7056.178 Da.

1

PRO

GLU

VAL

ILE

SER

LYS

ILE

MET

GLU

2

ASN

PRO

ASP

VAL

ALA

MET

ALA

PHE

GLN

ASN

3

PRO

ARG

VAL

GLN

ALA

LEU

MET

GLU

CYS

4

SER

GLU

ASN

PRO

MET

ASN

ILE

MET

LYS

TYR

5

GLN

ASN

ASP

LYS

GLU

VAL

MET

ASP

VAL

PHE

6

ASN

LYS

ILE

SER

GLN

LEU

PHE

PRO

GLY

MET

7

THR

GLY

Samples:

Tic40-Hip-Hop: potassium phosphate 20 mM; sodium citrate 20 mM; sodium chloride 100 mM; TFE, [U-99% 2H], 10%; H2O 90%; D2O 10%

Tic40-Hip-Hop_NMR_sample: ionic strength: 140 mM; pH: 5.56; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample
2D 1H-13C HSQC	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample
3D CBCA(CO)NH	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample
3D HNCACB	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample
3D HNCO	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample
3D HN(CA)CO	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample
3D HNCA	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample
3D HN(CO)CA	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample
3D HCCH-TOCSY	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample
3D CCCONH	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample
3D 1H-15N NOESY	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample
3D 1H-15N TOCSY	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample
3D 1H-13C NOESY	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample
2D 1H-1H NOESY	Tic40-Hip-Hop	isotropic	Tic40-Hip-Hop_NMR_sample

Software:

X-PLOR NIH v2.18, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMRView v5, Johnson, One Moon Scientific - chemical shift assignment, peak picking

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

Bruker Avance 600 MHz

PDB	2LNM
DBJ	BAB10189
EMBL	CDX69438 CDX85495 CDY38098
GB	AAL16131 AAM13009 AAP31939 AED92318 EFH47986
REF	NP_197165 XP_002871727 XP_006287761 XP_006287762 XP_006400200
SP	Q9FMD5
AlphaFold	Q9FMD5