BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 18190

Title: NMR structure of the protein BC008182, a DNAJ-like domain from Homo sapiens

Deposition date: 2012-01-09 Original release date: 2012-02-13

Authors: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt; JCSG, JCSG

Citation: Dutta, Samit; Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt; JCSG, JCSG. "NMR structure of the protein BC008182, DNAJ homolog from Homo sapiens"  .

Assembly members:
entity, polymer, 71 residues, 8224.376 Da.

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pSpeedet

Entity Sequences (FASTA):
entity: GMVKETTYYDVLGVKPNATQ EELKKAYRKLALKYHPDKNP NEGEKFKQISQAYEVLSDAK KRELYDKGGEQ

Data sets:
Data typeCount
13C chemical shifts249
15N chemical shifts75
1H chemical shifts498

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BC0081821

Entities:

Entity 1, BC008182 71 residues - 8224.376 Da.

1   GLYMETVALLYSGLUTHRTHRTYRTYRASP
2   VALLEUGLYVALLYSPROASNALATHRGLN
3   GLUGLULEULYSLYSALATYRARGLYSLEU
4   ALALEULYSTYRHISPROASPLYSASNPRO
5   ASNGLUGLYGLULYSPHELYSGLNILESER
6   GLNALATYRGLUVALLEUSERASPALALYS
7   LYSARGGLULEUTYRASPLYSGLYGLYGLU
8   GLN

Samples:

sample_1: entity, [U-98% 13C; U-98% 15N], 1.2 mM; sodium phosphate 20 mM; sodium azide 5 mM; sodium chloride 50 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.080 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
4D HACANH APSYsample_1isotropicsample_conditions_1
5D HACACONH APSYsample_1isotropicsample_conditions_1
5D CBCACONH APSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1

Software:

CYANA v3.0, G ntert P. - structure solution

TOPSPIN v2.1, Bruker - collection, processing

CARA, Keller and Wuthrich - chemical shift assignment, data analysis

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

BMRB 19163
PDB
DBJ BAA02656 BAC38744 BAC82111 BAD82815 BAE26788
EMBL CAI29674
GB AAA98855 AAC37517 AAC78597 AAH08182 AAH57876
PIR S34632
REF NP_001015637 NP_001127102 NP_001158143 NP_001158144 NP_001231092
SP P31689 P63036 P63037 Q5E954 Q5NVI9
TPG DAA12974 DAA26671
AlphaFold P63037 P31689 P63036 Q5E954 Q5NVI9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts