BMRB Entry 18242

Name: Backbone and sidechain 1H, 15N and 13C assignments of NLRP10 (PYNOD) pyrin domain
Published: 2012-06-05

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PDB ID: 2m5v
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18242
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and sidechain 1H, 15N and 13C assignments of NLRP10 (PYNOD) pyrin domain

Deposition date:

2012-02-07

Original release date:

2012-06-05

Authors:

Chang, Chung-I; Chang, Chi-Fon; Su, Ming-Yuan

Citation:

Citation: Su, Ming-Yuan; Chang, Chung-I; Chang, Chi-Fon. "1H, 13C and 15N resonance assignments of the pyrin domain from human PYNOD." Biomol. NMR Assignments 7, 141-143 (2013).
PubMed: 22618865

Assembly members:

Assembly members:
PYNOD_PYD, polymer, 102 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PYNOD_PYD: GAMAMAKARKPREALLWALS DLEENDFKKLKFYLRDMTLS EGQPPLARGELEGLIPVDLA ELLISKYGEKEAVKVVLKGL KVMNLLELVDQLSHICLHDY RE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	428
15N chemical shifts	93
1H chemical shifts	719

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PYNOD PYD	1

Entities:

Entity 1, PYNOD PYD 102 residues - Formula weight is not available

1

GLY

ALA

MET

ALA

MET

ALA

LYS

ALA

ARG

LYS

2

PRO

ARG

GLU

ALA

LEU

TRP

ALA

LEU

SER

3

ASP

LEU

GLU

ASN

ASP

PHE

LYS

LEU

4

LYS

PHE

TYR

LEU

ARG

ASP

MET

THR

LEU

SER

5

GLU

GLY

GLN

PRO

LEU

ALA

ARG

GLY

GLU

6

LEU

GLU

GLY

LEU

ILE

PRO

VAL

ASP

LEU

ALA

7

GLU

LEU

ILE

SER

LYS

TYR

GLY

GLU

LYS

8

GLU

ALA

VAL

LYS

VAL

LEU

LYS

GLY

LEU

9

LYS

VAL

MET

ASN

LEU

GLU

LEU

VAL

ASP

10

GLN

LEU

SER

HIS

ILE

CYS

LEU

HIS

ASP

TYR

11

ARG

GLU

Samples:

sample_1: PYNOD PYD, [U-13C; U-15N], 0.2 mM

sample_conditions_1: ionic strength: 140 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

PDB	2M5V
GB	AAI04958 AAO18161 AAS67384 AIC53619 EAW68640
REF	NP_789791 XP_003254944 XP_003313013 XP_003818292 XP_004050717
SP	Q86W26
TPG	DAA01243
AlphaFold	Q86W26