BMRB Entry 18244

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PDB ID: 2lpb
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18244
MolProbity Validation Chart

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Title:
Structure of the complex of the central activation doamin of Gcn4 bound to the mediator co-activator domain 1 of Gal11/med15
Deposition date:
2012-02-07
Original release date:
2012-03-28
Authors:
Brzovic, Peter; Heikaus, Clemens; Kisselev, Leonid; Vernon, Robert; Herbig, Eric; Pacheco, Derek; Warfield, Linda; Littlefield, Peter; Baker, David; Klevit, Rachel; Hahn, Steven
Citation:

Citation: Brzovic, Peter; Heikaus, Clemens; Kisselev, Leonid; Vernon, Robert; Herbig, Eric; Pacheco, Derek; Warfield, Linda; Baker, David; Klevit, Rachel; Hahn, Steven. "The Acidic Transcription Activator Gcn4 Binds the Mediator Subunit Gal11/Med15 Using a Simple Protein Interface Forming a Fuzzy Complex"  Mol. Cell 44, 942-953 (2011).
PubMed: 22195967

Assembly members:

Assembly members:
entity_1, polymer, 81 residues, 9514.164 Da.
entity_2, polymer, 34 residues, 3958.110 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: QRRQLTPQQQQLVNQMKVAP IPKQLLQRIPNIPPNINTWQ QVTALAQQKLLTPQDMEAAK QVYKIHQQLLFKARLQQQQA Q
entity_2: STDSTPMFEYENLEDNSKEW TSLFDNDIPVTTDD

Data sets:
Data typeCount
13C chemical shifts487
15N chemical shifts102
1H chemical shifts761

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1central activation doamin of Gcn41
2domain 1 of Gal11/med152

Entities:

Entity 1, central activation doamin of Gcn4 81 residues - 9514.164 Da.

1   GLNARGARGGLNLEUTHRPROGLNGLNGLN
2   GLNLEUVALASNGLNMETLYSVALALAPRO
3   ILEPROLYSGLNLEULEUGLNARGILEPRO
4   ASNILEPROPROASNILEASNTHRTRPGLN
5   GLNVALTHRALALEUALAGLNGLNLYSLEU
6   LEUTHRPROGLNASPMETGLUALAALALYS
7   GLNVALTYRLYSILEHISGLNGLNLEULEU
8   PHELYSALAARGLEUGLNGLNGLNGLNALA
9   GLN

Entity 2, domain 1 of Gal11/med15 34 residues - 3958.110 Da.

1   SERTHRASPSERTHRPROMETPHEGLUTYR
2   GLUASNLEUGLUASPASNSERLYSGLUTRP
3   THRSERLEUPHEASPASNASPILEPROVAL
4   THRTHRASPASP

Related Database Links:

PDB 2LPB
DBJ GAA26277 GAA22815
EMBL CAA62537 CAA99056 CAY86238 CAE52206 CAE52207 CAE52208 CAE52209 CAE52210
GB AAA34622 AHY77262 AJP41494 AJT70913 AJT71404 AAA34640 AAA65521 AAB64486 AHY75543 EDN62959
REF NP_014591 NP_010907
SP P19659 P03069
TPG DAA10733 DAA07643
AlphaFold P19659 P03069

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SPARKY: Backbone or all simulated peaks