BMRB Entry 18438
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PDB ID: 2lso
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18438
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Eletsky, Alexander; Lee, Hsiau-Wei; Lee, Dan; Kohan, Eitan; Janjua, Haleema; Xiao, Rong; Acton, Thomas; Everett, John; Montelione, Gaetano; Prestegard, James; Szyperski, Thomas. "Solution NMR Structure of the Globular Domain of Human Histone H1x, Northeast Structural Genomics Consortium (NESG) Target HR7057A" To be published ., .-..
Assembly members:
HR7057A, polymer, 83 residues, 9400.875 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15Avi6HT_NESG
Entity Sequences (FASTA):
HR7057A: SHMQPGKYSQLVVETIRRLG
ERNGSSLAKIYTEAKKVPWF
DQQNGRTYLKYSIKALVQND
TLLQVKGTGANGSFKLNRKK
LEG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 372 |
| 15N chemical shifts | 88 |
| 1H chemical shifts | 603 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HR7057A | 1 |
Entities:
Entity 1, HR7057A 83 residues - 9400.875 Da.
Residues 4-83 correspond to the 44-123 fragment of the native H1x. Residues 1-3 represent the remainder of the affinity purification tag after TEV cleavage
| 1 | SER | HIS | MET | GLN | PRO | GLY | LYS | TYR | SER | GLN | ||||
| 2 | LEU | VAL | VAL | GLU | THR | ILE | ARG | ARG | LEU | GLY | ||||
| 3 | GLU | ARG | ASN | GLY | SER | SER | LEU | ALA | LYS | ILE | ||||
| 4 | TYR | THR | GLU | ALA | LYS | LYS | VAL | PRO | TRP | PHE | ||||
| 5 | ASP | GLN | GLN | ASN | GLY | ARG | THR | TYR | LEU | LYS | ||||
| 6 | TYR | SER | ILE | LYS | ALA | LEU | VAL | GLN | ASN | ASP | ||||
| 7 | THR | LEU | LEU | GLN | VAL | LYS | GLY | THR | GLY | ALA | ||||
| 8 | ASN | GLY | SER | PHE | LYS | LEU | ASN | ARG | LYS | LYS | ||||
| 9 | LEU | GLU | GLY |
Related Database Links:
| PDB | 2LSO |
| DBJ | BAA11018 BAE90932 |
| GB | AAH00426 AAH10435 EAW79264 EFB22594 ELK15204 |
| REF | NP_001253012 NP_001273421 NP_006017 XP_002697189 XP_002758712 |
| SP | Q92522 |
| TPG | DAA16748 |
| AlphaFold | Q92522 |
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