BMRB Entry 18462
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PDB ID: 2lt7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18462
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Buck-Koehntop, Bethany; Stanfield, Robyn; Ekiert, Damian; Martinez-Yamout, Maria; Dyson, H. Jane; Wilson, Ian; Wright, Peter. "Molecular basis for recognition of methylated and specific DNA sequences by the zinc finger protein Kaiso." Proc. Natl. Acad. Sci. U.S.A. 109, 15229-15234 (2012).
PubMed: 22949637
Assembly members:
Kaiso, polymer, 133 residues, 16086.681 Da.
DNA_strand_1, polymer, 19 residues, 5780.795 Da.
DNA_strand_2, polymer, 19 residues, 5869.857 Da.
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21d
Entity Sequences (FASTA):
Kaiso: ANKRMKVKHDDHYELIVDGR
VYYICIVCKRSYVCLTSLRR
HFNIHSWEKKYPCRYCEKVF
PLAEYRTKHEIHHTGERRYQ
CLACGKSFINYQFMSSHIKS
VHSQDPSGDSKLYRLHPCRS
LQIRQYAYLSDRS
DNA_strand_1: GTGCTTCCTGCCAATAACG
DNA_strand_2: CGTTATTGGCAGGAAGCAC
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 609 |
| 15N chemical shifts | 164 |
| 1H chemical shifts | 1288 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Kaiso | 1 |
| 2 | DNA_strand_1 | 2 |
| 3 | DNA_strand_2 | 3 |
| 4 | ZINC ION_1 | 4 |
| 5 | ZINC ION_2 | 4 |
| 6 | ZINC ION_3 | 4 |
Entities:
Entity 1, Kaiso 133 residues - 16086.681 Da.
| 1 | ALA | ASN | LYS | ARG | MET | LYS | VAL | LYS | HIS | ASP | ||||
| 2 | ASP | HIS | TYR | GLU | LEU | ILE | VAL | ASP | GLY | ARG | ||||
| 3 | VAL | TYR | TYR | ILE | CYS | ILE | VAL | CYS | LYS | ARG | ||||
| 4 | SER | TYR | VAL | CYS | LEU | THR | SER | LEU | ARG | ARG | ||||
| 5 | HIS | PHE | ASN | ILE | HIS | SER | TRP | GLU | LYS | LYS | ||||
| 6 | TYR | PRO | CYS | ARG | TYR | CYS | GLU | LYS | VAL | PHE | ||||
| 7 | PRO | LEU | ALA | GLU | TYR | ARG | THR | LYS | HIS | GLU | ||||
| 8 | ILE | HIS | HIS | THR | GLY | GLU | ARG | ARG | TYR | GLN | ||||
| 9 | CYS | LEU | ALA | CYS | GLY | LYS | SER | PHE | ILE | ASN | ||||
| 10 | TYR | GLN | PHE | MET | SER | SER | HIS | ILE | LYS | SER | ||||
| 11 | VAL | HIS | SER | GLN | ASP | PRO | SER | GLY | ASP | SER | ||||
| 12 | LYS | LEU | TYR | ARG | LEU | HIS | PRO | CYS | ARG | SER | ||||
| 13 | LEU | GLN | ILE | ARG | GLN | TYR | ALA | TYR | LEU | SER | ||||
| 14 | ASP | ARG | SER |
Entity 2, DNA_strand_1 19 residues - 5780.795 Da.
| 1 | DG | DT | DG | DC | DT | DT | DC | DC | DT | DG | ||||
| 2 | DC | DC | DA | DA | DT | DA | DA | DC | DG |
Entity 3, DNA_strand_2 19 residues - 5869.857 Da.
| 1 | DC | DG | DT | DT | DA | DT | DT | DG | DG | DC | ||||
| 2 | DA | DG | DG | DA | DA | DG | DC | DA | DC |
Entity 4, ZINC ION_1 - Zn - 65.409 Da.
| 1 | ZN |
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