BMRB Entry 18507

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PDB ID: 2lu3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18507
MolProbity Validation Chart

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Title:
Solution NMR structure of the apo-form of the beta2 carbohydrate module of AMP-activated protein kinase
Deposition date:
2012-06-08
Original release date:
2012-08-23
Authors:
Gooley, Paul; Koay, Ann; Stapleton, David
Citation:

Citation: Bieri, Michael; Mobbs, Jesse; Koay, Ann; Louey, Gavin; Mok, Yee-Foong; Hatters, Danny; Park, Jong-Tae; Park, Kwan-Hwa; Neumann, Dietbert; Stapleton, David; Gooley, Paul. "AMP-activated protein kinase beta-subunit requires internal motion for optimal carbohydrate binding."  Biophys. J. 102, 305-314 (2012).
PubMed: 22339867

Assembly members:

Assembly members:
entity, polymer, 105 residues, 11826.329 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pProEXHTc

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GAMGIRNSDSVKPTQQARPT VIRWSEGGKEVFISGSFNNW STKIPLIKSHNDFVAILDLP EGEHQYKFFVDGQWVHDPSE PVVTSQLGTINNLIHVKKSD FEVFD

Data sets:
Data typeCount
13C chemical shifts397
15N chemical shifts103
1H chemical shifts699

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1beta2 carbohydrate module of AMP-activated protein kinase1

Entities:

Entity 1, beta2 carbohydrate module of AMP-activated protein kinase 105 residues - 11826.329 Da.

1   GLYALAMETGLYILEARGASNSERASPSER
2   VALLYSPROTHRGLNGLNALAARGPROTHR
3   VALILEARGTRPSERGLUGLYGLYLYSGLU
4   VALPHEILESERGLYSERPHEASNASNTRP
5   SERTHRLYSILEPROLEUILELYSSERHIS
6   ASNASPPHEVALALAILELEUASPLEUPRO
7   GLUGLYGLUHISGLNTYRLYSPHEPHEVAL
8   ASPGLYGLNTRPVALHISASPPROSERGLU
9   PROVALVALTHRSERGLNLEUGLYTHRILE
10   ASNASNLEUILEHISVALLYSLYSSERASP
11   PHEGLUVALPHEASP

Related Database Links:

BMRB 18508
PDB 2F15 2LU3 2LU4 4RER 4REW 4Y0G 4YEE
DBJ BAE90075 BAF85509
EMBL CAA12030 CAH72644 CAH90491
GB AAF01293 AAH53610 AAH60228 AAH78821 AAM74153
REF NP_001075383 NP_001125257 NP_001179257 NP_001230612 NP_005390
SP O43741 Q6PAM0 Q9QZH4
TPG DAA31622
AlphaFold O43741 Q6PAM0 Q9QZH4

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