BMRB Entry 18540

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PDB ID: 2lut
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18540
MolProbity Validation Chart

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Title:
NMR solution structure of midkine-a
Deposition date:
2012-06-21
Original release date:
2013-04-02
Authors:
Lim, Jackwee; Yang, Daiwen; Meng, Dan
Citation:

Citation: Lim, Jackwee; Yao, Sheng; Graf, Martin; Winkler, Christoph; Yang, Daiwen. "Structure-function analysis of full-length midkine reveals novel residues important for heparin binding and zebrafish embryogenesis."  Biochem. J. 451, 407-415 (2013).
PubMed: 23418741

Assembly members:

Assembly members:
midkine-a, polymer, 125 residues, 13548.584 Da.

Natural source:

Natural source:   Common Name: Zebrafish   Taxonomy ID: 7955   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Danio rerio

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
midkine-a: GSKNKKEKNKGGKGGADCAE WLYGSCVANNGDCGQGMREG TCNEQTRKVKCRVPCNWKKE FGADCKYKFGNWGECDAATS TKSRTGTLQKALFNVECQQT VSVTKPCTTKVKNKPKGKKG KGKGN

Data sets:
Data typeCount
13C chemical shifts367
15N chemical shifts122
1H chemical shifts628

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1midkine-a1

Entities:

Entity 1, midkine-a 125 residues - 13548.584 Da.

1   GLYSERLYSASNLYSLYSGLULYSASNLYS
2   GLYGLYLYSGLYGLYALAASPCYSALAGLU
3   TRPLEUTYRGLYSERCYSVALALAASNASN
4   GLYASPCYSGLYGLNGLYMETARGGLUGLY
5   THRCYSASNGLUGLNTHRARGLYSVALLYS
6   CYSARGVALPROCYSASNTRPLYSLYSGLU
7   PHEGLYALAASPCYSLYSTYRLYSPHEGLY
8   ASNTRPGLYGLUCYSASPALAALATHRSER
9   THRLYSSERARGTHRGLYTHRLEUGLNLYS
10   ALALEUPHEASNVALGLUCYSGLNGLNTHR
11   VALSERVALTHRLYSPROCYSTHRTHRLYS
12   VALLYSASNLYSPROLYSGLYLYSLYSGLY
13   LYSGLYLYSGLYASN

Related Database Links:

PDB 2LUT
GB AAD38157 AAH71342 AAM27446 AJG05945
REF NP_571145

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