BMRB Entry 18565

Name: Backbone and side-chain resonance assignments of the membrane localization domain from Pasteurella multocida toxin
Published: 2014-02-11

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PDB ID: 2n9v
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18565
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side-chain resonance assignments of the membrane localization domain from Pasteurella multocida toxin

Deposition date:

2012-07-01

Original release date:

2014-02-11

Authors:

Brothers, Michael; Geissler, Brett; Hisao, Grant; Satchell, Karla; Wilson, Brenda; Rienstra, Chad

Citation:

Citation: Brothers, Michael; Geissler, Brett; Hisao, Grant; Satchell, Karla; Wilson, Brenda; Rienstra, Chad. "Backbone and side-chain resonance assignments of the membrane localization domain from Pasteurella multocida toxin." Biomol. NMR Assignments 8, 221-224 (2014).
PubMed: 23765284

Assembly members:

Assembly members:
MLD_PMT, polymer, 90 residues, 10.5 Da.

Natural source:

Natural source: Common Name: g-proteobacteria Taxonomy ID: 747 Superkingdom: Bacteria Kingdom: not available Genus/species: Pasteurella multocida

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pYC-pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MLD_PMT: MGVWTPEVLKARASVIGKPI GESYKRILAKLQRIHNSNIL DERQGLMHELMELIDLYEES QPSSERLNAFRELRTQLEKA LGLEHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	366
15N chemical shifts	81
1H chemical shifts	568

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MLD PMT	1

Entities:

Entity 1, MLD PMT 90 residues - 10.5 Da.

1	MET	GLY	VAL	TRP	THR	PRO	GLU	VAL	LEU	LYS
2	ALA	ARG	ALA	SER	VAL	ILE	GLY	LYS	PRO	ILE
3	GLY	GLU	SER	TYR	LYS	ARG	ILE	LEU	ALA	LYS
4	LEU	GLN	ARG	ILE	HIS	ASN	SER	ASN	ILE	LEU
5	ASP	GLU	ARG	GLN	GLY	LEU	MET	HIS	GLU	LEU
6	MET	GLU	LEU	ILE	ASP	LEU	TYR	GLU	GLU	SER
7	GLN	PRO	SER	SER	GLU	ARG	LEU	ASN	ALA	PHE
8	ARG	GLU	LEU	ARG	THR	GLN	LEU	GLU	LYS	ALA
9	LEU	GLY	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

PDB	2EBF 2EBH 2EC5
EMBL	CAA35885 CAA36717 CAA40921 CAA82233 CAD92744
GB	AAL55665 AAW57319 AAX76908 ABR23002 ACG56672
PRF	1703272A
REF	WP_015691094
SP	P17452
AlphaFold	P17452

BMRB Entry 18565

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links:

1	MET	GLY	VAL	TRP	THR	PRO	GLU	VAL	LEU	LYS
2	ALA	ARG	ALA	SER	VAL	ILE	GLY	LYS	PRO	ILE
3	GLY	GLU	SER	TYR	LYS	ARG	ILE	LEU	ALA	LYS
4	LEU	GLN	ARG	ILE	HIS	ASN	SER	ASN	ILE	LEU
5	ASP	GLU	ARG	GLN	GLY	LEU	MET	HIS	GLU	LEU
6	MET	GLU	LEU	ILE	ASP	LEU	TYR	GLU	GLU	SER
7	GLN	PRO	SER	SER	GLU	ARG	LEU	ASN	ALA	PHE
8	ARG	GLU	LEU	ARG	THR	GLN	LEU	GLU	LYS	ALA
9	LEU	GLY	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	GLY	VAL	TRP	THR	PRO	GLU	VAL	LEU	LYS
2	ALA	ARG	ALA	SER	VAL	ILE	GLY	LYS	PRO	ILE
3	GLY	GLU	SER	TYR	LYS	ARG	ILE	LEU	ALA	LYS
4	LEU	GLN	ARG	ILE	HIS	ASN	SER	ASN	ILE	LEU
5	ASP	GLU	ARG	GLN	GLY	LEU	MET	HIS	GLU	LEU
6	MET	GLU	LEU	ILE	ASP	LEU	TYR	GLU	GLU	SER
7	GLN	PRO	SER	SER	GLU	ARG	LEU	ASN	ALA	PHE
8	ARG	GLU	LEU	ARG	THR	GLN	LEU	GLU	LYS	ALA
9	LEU	GLY	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS

1	MET	GLY	VAL	TRP	THR	PRO	GLU	VAL	LEU	LYS
2	ALA	ARG	ALA	SER	VAL	ILE	GLY	LYS	PRO	ILE
3	GLY	GLU	SER	TYR	LYS	ARG	ILE	LEU	ALA	LYS
4	LEU	GLN	ARG	ILE	HIS	ASN	SER	ASN	ILE	LEU
5	ASP	GLU	ARG	GLN	GLY	LEU	MET	HIS	GLU	LEU
6	MET	GLU	LEU	ILE	ASP	LEU	TYR	GLU	GLU	SER
7	GLN	PRO	SER	SER	GLU	ARG	LEU	ASN	ALA	PHE
8	ARG	GLU	LEU	ARG	THR	GLN	LEU	GLU	LYS	ALA
9	LEU	GLY	LEU	GLU	HIS	HIS	HIS	HIS	HIS	HIS