BMRB Entry 18635
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18635
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Title: Backbone resonance assignments of human beta-defensin 1 PubMed: 24970887
Deposition date: 2012-08-02 Original release date: 2019-08-30
Authors: de Paula, Viviane; Gomes, Natalia; Lima, Luize; Miyamoto, Catarina; Monteiro, Robson; Almeida, Fabio; Valente, Ana Paula
Citation: De Paula, Viviane; Pomin, Vitor; Valente, Ana Paula. "Unique properties of human beta-defensin 6 (hBD6) and glycosaminoglycan complex: sandwich-like dimerization and competition with the chemokine receptor 2 (CCR2) binding site" J. Biol. Chem. 289, 22969-22979 (2014).
Assembly members:
HbD1, polymer, 36 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet32a
Entity Sequences (FASTA):
HbD1: DHYNCVSSGGQCLYSACPIF
TKIQGTCYRGKAKCCK
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 61 |
15N chemical shifts | 33 |
1H chemical shifts | 103 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | HbD1 | 1 |
Entities:
Entity 1, HbD1 36 residues - Formula weight is not available
1 | ASP | HIS | TYR | ASN | CYS | VAL | SER | SER | GLY | GLY | ||||
2 | GLN | CYS | LEU | TYR | SER | ALA | CYS | PRO | ILE | PHE | ||||
3 | THR | LYS | ILE | GLN | GLY | THR | CYS | TYR | ARG | GLY | ||||
4 | LYS | ALA | LYS | CYS | CYS | LYS |
Samples:
sample_1: HbD1 0.5 mM; sodium phosphate0.2 0.6 mM; H2O 90%; D2O 10%; phosphate buffer 30 mM; sodium chloride 50 mM; potassium chloride 0.9 mM
sample_conditions_1: ionic strength: 20 M; pH: 7.4; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts