BMRB Entry 18657
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PDB ID: 2lx2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18657
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Kumeta, Hiroyuki; Ogura, Kenji; Nishimiya, Yoshiyuki; Miura, Ai; Inagaki, Fuyuhiko; Tsuda, Sakae. "NMR structure note: a defective isoform and its activity-improved variant of a type III antifreeze protein from Zoarces elongates Kner." J. Biomol. NMR 55, 225-230 (2013).
PubMed: 23288342
Assembly members:
nfeAFP11, polymer, 67 residues, 7071.434 Da.
Natural source: Common Name: not available Taxonomy ID: 291231 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Zoarces elongatus
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pKK223-3UC
Entity Sequences (FASTA):
nfeAFP11: MNQESVVAAQLIPINTALTL
VMMTTRVVSPTGIPAEDIPR
LISMQVNQVVPMGTTLMPDM
VKGYAPA
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 292 |
| 15N chemical shifts | 67 |
| 1H chemical shifts | 511 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | nfeAFP11 | 1 |
Entities:
Entity 1, nfeAFP11 67 residues - 7071.434 Da.
| 1 | MET | ASN | GLN | GLU | SER | VAL | VAL | ALA | ALA | GLN | ||||
| 2 | LEU | ILE | PRO | ILE | ASN | THR | ALA | LEU | THR | LEU | ||||
| 3 | VAL | MET | MET | THR | THR | ARG | VAL | VAL | SER | PRO | ||||
| 4 | THR | GLY | ILE | PRO | ALA | GLU | ASP | ILE | PRO | ARG | ||||
| 5 | LEU | ILE | SER | MET | GLN | VAL | ASN | GLN | VAL | VAL | ||||
| 6 | PRO | MET | GLY | THR | THR | LEU | MET | PRO | ASP | MET | ||||
| 7 | VAL | LYS | GLY | TYR | ALA | PRO | ALA |
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