BMRB Entry 18749

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PDB ID: 2lze
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18749
MolProbity Validation Chart

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Title:
Ligase 10C
Deposition date:
2012-10-01
Original release date:
2012-11-02
Authors:
Chao, Fa-An; Morelli, Aleardo; Haugner, John; Churchfield, Lewis; Hagmann, Leonardo; Shi, Lei; Masterson, Larry; Sarangi, Ritimukta; Veglia, Gianluigi; Seelig, Burckhard
Citation:

Citation: Chao, Fa-An; Morelli, Aleardo; Iii, John C Haugner; Churchfield, Lewis; Hagmann, Leonardo; Shi, Lei; Masterson, Larry; Sarangi, Ritimukta; Veglia, Gianluigi; Seelig, Burckhard. "Structure and dynamics of a primordial catalytic fold generated by in vitro evolution."  Nat. Chem. Biol. 9, 81-83 (2013).
PubMed: 23222886

Assembly members:

Assembly members:
entity, polymer, 87 residues, 9795.925 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MGAPVPYPDPLEPRGGKHIC AICGNNAEDYKHTDMDLTYT DRDYKNCESYHKCSDLCQYC RYQKDLAIHHQHHHGGSMGM SGSGTGY

Data sets:
Data typeCount
13C chemical shifts227
15N chemical shifts61
1H chemical shifts320

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Ligase 10C1
2Zinc Ion2

Entities:

Entity 1, Ligase 10C 87 residues - 9795.925 Da.

1   METGLYALAPROVALPROTYRPROASPPRO
2   LEUGLUPROARGGLYGLYLYSHISILECYS
3   ALAILECYSGLYASNASNALAGLUASPTYR
4   LYSHISTHRASPMETASPLEUTHRTYRTHR
5   ASPARGASPTYRLYSASNCYSGLUSERTYR
6   HISLYSCYSSERASPLEUCYSGLNTYRCYS
7   ARGTYRGLNLYSASPLEUALAILEHISHIS
8   GLNHISHISHISGLYGLYSERMETGLYMET
9   SERGLYSERGLYTHRGLYTYR

Entity 2, Zinc Ion - Zn - 65.409 Da.

1   ZN

Related Database Links:

PDB 2LZE

Download HSQC peak lists in one of the following formats:
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