BMRB Entry 18826
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PDB ID: 2m0v
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18826
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Bhattacharya, Shibani; Ju, Jeong Ho; Orlova, Natalia; Khajeh, Jahan Ali; Cowburn, David; Bu, Zimei. "Ligand-Induced Dynamic Changes in Extended PDZ Domains from NHERF1." J. Mol. Biol. 425, 2509-2528 (2013).
PubMed: 23583913
Assembly members:
PDZ2, polymer, 128 residues, 14101.081 Da.
CFTR, polymer, 5 residues, 632.693 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET151/D-TOPO
Entity Sequences (FASTA):
PDZ2: GIDPFTMLRPRLCTMKKGPS
GYGFNLHSDKSKPGQFIRSV
DPDSPAEASGLRAQDRIVEV
NGVCMEGKQHGDVVSAIRAG
GDETKLLVVDRETDEFFKKC
RVIPSQEHLNGPLPVPFTNG
EIQKENSR
CFTR: QDTRL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 508 |
| 15N chemical shifts | 132 |
| 1H chemical shifts | 894 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | extended PDZ2 domain from NHERF1 | 1 |
| 2 | C-terminal CFTR peptide | 2 |
Entities:
Entity 1, extended PDZ2 domain from NHERF1 128 residues - 14101.081 Da.
| 1 | GLY | ILE | ASP | PRO | PHE | THR | MET | LEU | ARG | PRO | ||||
| 2 | ARG | LEU | CYS | THR | MET | LYS | LYS | GLY | PRO | SER | ||||
| 3 | GLY | TYR | GLY | PHE | ASN | LEU | HIS | SER | ASP | LYS | ||||
| 4 | SER | LYS | PRO | GLY | GLN | PHE | ILE | ARG | SER | VAL | ||||
| 5 | ASP | PRO | ASP | SER | PRO | ALA | GLU | ALA | SER | GLY | ||||
| 6 | LEU | ARG | ALA | GLN | ASP | ARG | ILE | VAL | GLU | VAL | ||||
| 7 | ASN | GLY | VAL | CYS | MET | GLU | GLY | LYS | GLN | HIS | ||||
| 8 | GLY | ASP | VAL | VAL | SER | ALA | ILE | ARG | ALA | GLY | ||||
| 9 | GLY | ASP | GLU | THR | LYS | LEU | LEU | VAL | VAL | ASP | ||||
| 10 | ARG | GLU | THR | ASP | GLU | PHE | PHE | LYS | LYS | CYS | ||||
| 11 | ARG | VAL | ILE | PRO | SER | GLN | GLU | HIS | LEU | ASN | ||||
| 12 | GLY | PRO | LEU | PRO | VAL | PRO | PHE | THR | ASN | GLY | ||||
| 13 | GLU | ILE | GLN | LYS | GLU | ASN | SER | ARG |
Entity 2, C-terminal CFTR peptide 5 residues - 632.693 Da.
| 1 | GLN | ASP | THR | ARG | LEU |
Related Database Links:
| BMRB | 15567 16637 16638 |
| PDB | 2JXO 2KJD 2KRG 2M0V 2OZF 4Q3H |
| DBJ | BAG54683 |
| GB | EAW89189 EHH58322 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks