BMRB Entry 18831

Title:
Solution structure of U14Ub1, an engineered ubiquitin variant with increased affinity for USP14
Deposition date:
2012-11-08
Original release date:
2013-06-24
Authors:
Phillips, Aaron; Fairbrother, Wayne; Corn, Jacob
Citation:

Citation: Phillips, Aaron; Zhang, Yingnan; Cunningham, Christian; Zhou, Lijuan; Forrest, William; Liu, Peter; Steffek, Micah; Lee, James; Tam, Christine; Helgason, Elizabeth; Murray, Jeremy; Kirkpatrick, Donald; Fairbrother, Wayne; Corn, Jacob. "Conformational dynamics control ubiquitin-deubiquitinase interactions and influence in vivo signaling."  Proc. Natl. Acad. Sci. U.S.A. 110, 11379-11384 (2013).
PubMed: 23801757

Assembly members:

Assembly members:
entity, polymer, 78 residues, 8565.851 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-based

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts328
15N chemical shifts70
1H chemical shifts518

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1U14Ub11

Entities:

Entity 1, U14Ub1 78 residues - 8565.851 Da.

1   GLYSERMETGLNILEPHEVALLYSGLYLEU
2   THRGLYLYSTHRTHRTHRLEUGLUVALGLU
3   PROSERASPTHRILEGLUASNVALLYSALA
4   LYSILEGLNASPLYSTHRGLYLEUPROPRO
5   ASPGLNGLNARGLEUILEPHEALAGLYLYS
6   GLNLEUGLUASPGLYARGTHRLEUSERASP
7   TYRASNILEGLNLYSGLUSERTHRLEUHIS
8   ILEVALTRPARGLEUARGGLYGLY

Related Database Links:

PDB 2M0X

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