BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 18844

Title: ubiquitin-like domain-containing C-terminal domain phosphatase (UBLCP1)   PubMed: 23667555

Deposition date: 2012-11-19 Original release date: 2013-05-30

Authors: Lee, Weontae; Yun, Ji-Hye

Citation: Yun, Ji-Hye; Ko, Sunggeon; Lee, Chung-Kyung; Cheong, Hae-Kap; Cheong, Chaejoon; Yoon, Jong-Bok; Lee, Weontae. "Solution Structure and Rpn1 Interaction of the UBL Domain of Human RNA Polymerase II C-Terminal Domain Phosphatase."  PLoS ONE 8, e62981-e62981 (2013).

Assembly members:
entity_1, polymer, 82 residues, 8997.747 Da.
PL, non-polymer, Formula weight is not available
entity_LL5, non-polymer, 334.436 Da.
ORI, non-polymer, Formula weight is not available

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX 4T-1

Entity Sequences (FASTA):
entity_1: MALPIIVKWGGQEYSVTTLS EDDTVLDLKQFLKTLTGVLP ERQKLLGLKVKGKPAENDVK LGALKLKPNTKIMMMGTREE SX

Data sets:
Data typeCount
13C chemical shifts258
15N chemical shifts77
1H chemical shifts565

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UBLCP11
2PL2
3LL5_13
4LL5_23
5LL5_33
6LL5_43
7LL5_53
8ORI4

Entities:

Entity 1, UBLCP1 82 residues - 8997.747 Da.

1   METALALEUPROILEILEVALLYSTRPGLY
2   GLYGLNGLUTYRSERVALTHRTHRLEUSER
3   GLUASPASPTHRVALLEUASPLEULYSGLN
4   PHELEULYSTHRLEUTHRGLYVALLEUPRO
5   GLUARGGLNLYSLEULEUGLYLEULYSVAL
6   LYSGLYLYSPROALAGLUASNASPVALLYS
7   LEUGLYALALEULYSLEULYSPROASNTHR
8   LYSILEMETMETMETGLYTHRARGGLUGLU
9   SERNH2

Entity 2, PL - Formula weight is not available

Entity 3, LL5_1 - C16 H22 N4 O2 S - 334.436 Da.

1   LL5

Entity 4, ORI - Formula weight is not available

Samples:

sample_1: entity_1, [U-15N], 0.6 mM; entity_1, [U-13C; U-15N], 0.6 mM

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

TALOS, Cornilescu, Delaglio and Bax - data analysis

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker Avance 900 MHz

Related Database Links:

BMRB 16899 16908 17805
PDB
DBJ BAB71628 BAC31711 BAC34039 BAE28110 BAI46037
EMBL CAH93392
GB AAH13425 AAH85111 AAH89210 AAI05395 AAI06094
REF NP_001014139 NP_001039459 NP_001126990 NP_001253320 NP_077795
SP Q2KJD7 Q5FWT7 Q5R4C4 Q8BGR9 Q8WVY7
TPG DAA27217
AlphaFold Q8WVY7 Q2KJD7 Q5FWT7 Q5R4C4 Q8BGR9

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts