BMRB Entry 18850

Name: Solution structure of the Haloferax volcanii HVO_2177 protein
Published: 2013-08-05

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PDB ID: 2m19
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18850
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Haloferax volcanii HVO_2177 protein

Deposition date:

2012-11-21

Original release date:

2013-08-05

Authors:

Li, Yunfeng; Maciejewski, Mark; Martin, Jonathan; Jin, Kai; Zhang, Yuhang; Lu, Min; Maupin-Furlow, Julie; Hao, Bing

Citation:

Citation: Li, Yunfeng; Maciejewski, Mark; Martin, Jonathan; Jin, Kai; Zhang, Yuhang; Maupin-Furlow, Julie; Hao, Bing. "Crystal structure of the ubiquitin-like small archaeal modifier protein 2 from Haloferax volcanii." Protein Sci. 22, 1206-1217 (2013).
PubMed: 23821306

Assembly members:

Assembly members:
Ubl_protein_HVO_2177, polymer, 106 residues, 11540.825 Da.

Natural source:

Natural source: Common Name: Haloferax volcanii Taxonomy ID: 2246 Superkingdom: Archaea Kingdom: not available Genus/species: Haloferax volcanii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ubl_protein_HVO_2177: GGGRDYKDDDDKGTMELELR FFATFREVVGQKSIYWRVDD DATVGDVLRSLEAEYDGLAG RLIEDGEVKPHVNVLKNGRE VVHLDGMATALDDGDAVSVF PPVAGG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	441
15N chemical shifts	98
1H chemical shifts	678

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Haloferax volcanii HVO_2177 protein	1

Entities:

Entity 1, Haloferax volcanii HVO_2177 protein 106 residues - 11540.825 Da.

residues 1-14 represent a non-native affinity tag

1

GLY

ARG

ASP

TYR

LYS

ASP

2

ASP

LYS

GLY

THR

MET

GLU

LEU

GLU

LEU

ARG

3

PHE

ALA

THR

PHE

ARG

GLU

VAL

GLY

4

GLN

LYS

SER

ILE

TYR

TRP

ARG

VAL

ASP

5

ASP

ALA

THR

VAL

GLY

ASP

VAL

LEU

ARG

SER

6

LEU

GLU

ALA

GLU

TYR

ASP

GLY

LEU

ALA

GLY

7

ARG

LEU

ILE

GLU

ASP

GLY

GLU

VAL

LYS

PRO

8

HIS

VAL

ASN

VAL

LEU

LYS

ASN

GLY

ARG

GLU

9

VAL

HIS

LEU

ASP

GLY

MET

ALA

THR

ALA

10

LEU

ASP

GLY

ASP

ALA

VAL

SER

VAL

PHE

11

PRO

VAL

ALA

GLY

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_2	isotropic	sample_conditions_1

GB	8923994 ADE02783 ELK45920 ELY33406 ELZ55725 ELZ67244
PDB	2M19
EMBL	CQR48598
REF	WP_004042069 WP_006601267
SP	D4GVB0
AlphaFold	D4GVB0

BMRB Entry 18850

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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