BMRB Entry 18879

Title:
SP-B C-terminal (residues 59-80) peptide in DPC micelles
Deposition date:
2012-12-06
Original release date:
2013-12-16
Authors:
Kuznetsova, Anna; Vanni, Julieann; Long, Joanna
Citation:

Citation: Kuznetsova, Anna; Vanni, Julieann; Long, Joanna. "Solution NMR structures of the C-terminal segment of surfactant protein B (residues 59-80) in DPC detergent micelles and methanol."  Biochemistry ., .-..

Assembly members:

Assembly members:
entity, polymer, 22 residues, 2500.072 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET31b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: DTLLGRILPQLVCRLVLRCS ID

Data sets:
Data typeCount
15N chemical shifts24
1H chemical shifts173

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SP-B1

Entities:

Entity 1, SP-B 22 residues - 2500.072 Da.

there is homoserine lactone residue (HSL) at the end of the sequence after D80 that is left due to cyanogen bromide cleavage of the peptide from fusion construct at the methionines before D59 and after D80

1   ASPTHRLEULEUGLYARGILELEUPROGLN
2   LEUVALCYSARGLEUVALLEUARGCYSSER
3   ILEASP

Related Database Links:

PDB 2M1T

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks