BMRB Entry 18880

Name: SOLUTION STRUCTURE OF THE SMALL DICTYOSTELIUM DISCOIDEIUM MYOSIN LIGHT CHAIN MlcB PROVIDES INSIGHTS INTO IQ-MOTIF RECOGNITION OF CLASS I MYOSIN MYO1B
Published: 2013-12-09

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PDB ID: 2m1u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18880
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

SOLUTION STRUCTURE OF THE SMALL DICTYOSTELIUM DISCOIDEIUM MYOSIN LIGHT CHAIN MlcB PROVIDES INSIGHTS INTO IQ-MOTIF RECOGNITION OF CLASS I MYOSIN MYO1B

Deposition date:

2012-12-06

Original release date:

2013-12-09

Authors:

Liburd, Janine; Chitayat, Seth; Crawley, Scott; Denis, Christopher; Cote, Graham; Smith, Steven

Citation:

Citation: Liburd, Janine; Chitayat, Seth; Crawley, Scott; Munro, Kim; Miller, Emily; Denis, Chris; Spencer, Holly; Cote, Graham; Smith, Steven. "Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ motif Recognition." J. Biol. Chem. ., .-. (2014).
PubMed: 24790102

Assembly members:

Assembly members:
MlcB, polymer, 93 residues, 10471.464 Da.

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MlcB: MGSSHHHHHHSSGLVPRGSH MSDEKTQLIEAFYNFDGDYD GFVSVEEFRGIIRDGLPMTE AEITEFFEAADPNNTGFIDY KAFAAMLYSVDES

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	322
15N chemical shifts	85
1H chemical shifts	519

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MlcB	1

Entities:

Entity 1, MlcB 93 residues - 10471.464 Da.

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

SER

ASP

GLU

LYS

THR

GLN

LEU

ILE

GLU

4

ALA

PHE

TYR

ASN

PHE

ASP

GLY

ASP

TYR

ASP

5

GLY

PHE

VAL

SER

VAL

GLU

PHE

ARG

GLY

6

ILE

ARG

ASP

GLY

LEU

PRO

MET

THR

GLU

7

ALA

GLU

ILE

THR

GLU

PHE

GLU

ALA

8

ASP

PRO

ASN

THR

GLY

PHE

ILE

ASP

TYR

9

LYS

ALA

PHE

ALA

MET

LEU

TYR

SER

VAL

10

ASP

GLU

SER

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1

PDB	2M1U
GB	EAL62399 EGC36044
REF	XP_003287419 XP_635900
SP	Q54GL7
AlphaFold	Q54GL7

BMRB Entry 18880

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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