BMRB Entry 19014

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PDB ID: 2m4n
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19014
MolProbity Validation Chart

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Title:
Solution structure of the putative Ras interaction domain of AFD-1, isoform a from Caenorhabditis elegans
Deposition date:
2013-02-07
Original release date:
2013-03-04
Authors:
Harris, R.; Hillerich, B.; Ahmed, M.; Bonanno, J.; Chamala, S.; Evans, B.; Lafleur, J.; Hammonds, J.; Washington, E.; Stead, M.; Love, J.; Attonito, J.; Seidel, R.; Liddington, R.; Weis, W.; Nelson, W.; Girvin, M.; Almo, S.
Citation:

Citation: Harris, R.; Hillerich, B.; Ahmed, M.; Bonanno, J.; Chamala, S.; Evans, B.; Lafleur, J.; Hammonds, J.; Washington, E.; Stead, M.; Love, J.; Attonito, J.; Seidel, R.; Chook, Y.; Rout, M.; Liddington, R.; Weis, W.; Nelson, W.; Girvin, M.; Almo, S.. "Solution structure of the putative Ras interaction domain of AFD-1, isoform a from Caenorhabditis elegans"  To be published ., .-..

Assembly members:

Assembly members:
putative_Ras_interaction_domain_of_AFD-1_isoform_a, polymer, 108 residues, 11972.540 Da.

Natural source:

Natural source:   Common Name: Caenorhabditis elegans   Taxonomy ID: 6239   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: modified pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
putative_Ras_interaction_domain_of_AFD-1_isoform_a: SMFGGSLKVYGGEIVPTRPY VSILAEINENADRILGAALE KYGLEHSKDDFILVEVSNDD DRKSMSDLREIDGRPIPPTE CPLFEMTARSGNGENGFDSF LAIKRKPH

Data sets:
Data typeCount
13C chemical shifts478
15N chemical shifts108
1H chemical shifts751

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1putative Ras interaction domain of AFD-1, isoform a from Caenorhabditis elegans1

Entities:

Entity 1, putative Ras interaction domain of AFD-1, isoform a from Caenorhabditis elegans 108 residues - 11972.540 Da.

1   SERMETPHEGLYGLYSERLEULYSVALTYR
2   GLYGLYGLUILEVALPROTHRARGPROTYR
3   VALSERILELEUALAGLUILEASNGLUASN
4   ALAASPARGILELEUGLYALAALALEUGLU
5   LYSTYRGLYLEUGLUHISSERLYSASPASP
6   PHEILELEUVALGLUVALSERASNASPASP
7   ASPARGLYSSERMETSERASPLEUARGGLU
8   ILEASPGLYARGPROILEPROPROTHRGLU
9   CYSPROLEUPHEGLUMETTHRALAARGSER
10   GLYASNGLYGLUASNGLYPHEASPSERPHE
11   LEUALAILELYSARGLYSPROHIS

Related Database Links:

UNP Q9BIC1
PDB 2M4N
EMBL CCD69313 CCD69315 CCD69316 CDO41086
GB AAD20441
REF NP_001021660 NP_001021661 NP_001293329 NP_490938
AlphaFold Q9N400

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