BMRB Entry 19018

Title:
NMR structure of E. coli ribosomela decoding site with apramycin
Deposition date:
2013-02-10
Original release date:
2013-03-13
Authors:
Puglisi, Joseph; Tsai, Albert; Marshall, R. Andrew; Viani, Elisabetta
Citation:

Citation: Tsai, Albert; Uemura, Sotaro; Johansson, Magnus; Puglisi, Elisabetta Viani; Marshall, R. Andrew; Aitken, Colin Echeverria; Korlach, Jonas; Ehrenberg, Mans; Puglisi, Joseph. "The impact of aminoglycosides on the dynamics of translation elongation."  Cell Rep. 3, 497-508 (2013).
PubMed: 23416053

Assembly members:

Assembly members:
RNA, polymer, 27 residues, 132.116 Da.
APRAMYCIN, non-polymer, 539.577 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: enzymatic semisynthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA: GGCGUCACACCUUCGGGUGA AGUCGCC

Data sets:
Data typeCount
13C chemical shifts151
15N chemical shifts8
1H chemical shifts214

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA1
2APRAMYCIN_12
3APRAMYCIN_22
4APRAMYCIN_32

Entities:

Entity 1, RNA 27 residues - 132.116 Da.

1   GGCGUCACAC
2   CUUCGGGUGA
3   AGUCGCC

Entity 2, APRAMYCIN_1 - C21 H41 N5 O11 - 539.577 Da.

1   AM2