BMRB Entry 19023

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PDB ID: 2m4v
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19023
MolProbity Validation Chart

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Title:
Solution structure and sigma factor interactions of Mycobacterium tuberculosis RNA polymerase binding protein A
Deposition date:
2013-02-11
Original release date:
2013-04-11
Authors:
Bortoluzzi, Alessio; Muskett, Frederick; Waters, Lorna; Adiss, Philip; Rieck, Barbara; Munder, Thomas; Schleier, Susanne; Forti, Francesca; Ghisotti, Daniela; Carr, Mark; O'Hare, Helen
Citation:

Citation: Bortoluzzi, Alessio; Muskett, Frederick; Waters, Lorna; Adiss, Philip; Rieck, Barbara; Munder, Thomas; Schleier, Susanne; Forti, Francesca; Ghisotti, Daniela; Carr, Mark; O'Hare, Helen. "Structural characterization of Mycobacterium tuberculosis RNA polymerase binding protein A (RbpA) and its interactions with sigma factors"  J. Biol. Chem. 288, 14438-14450 (2013).
PubMed: 23548911

Assembly members:

Assembly members:
entity, polymer, 80 residues, 9026.145 Da.

Natural source:

Natural source:   Common Name: Mycobacterium tuberculosis   Taxonomy ID: 1773   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Mycobacterium tuberculosis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-43.1a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: SMADRVLRGSRLGAVSYETD RNHDLAPRQIARYRTDNGEE FEVPFADDAEIPGTWLCRNG MEGTLIEGDLPEPKKVKPPR

Data sets:
Data typeCount
13C chemical shifts252
15N chemical shifts76
1H chemical shifts485

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA polymerase binding protein A (RbpA)1

Entities:

Entity 1, RNA polymerase binding protein A (RbpA) 80 residues - 9026.145 Da.

1   SERMETALAASPARGVALLEUARGGLYSER
2   ARGLEUGLYALAVALSERTYRGLUTHRASP
3   ARGASNHISASPLEUALAPROARGGLNILE
4   ALAARGTYRARGTHRASPASNGLYGLUGLU
5   PHEGLUVALPROPHEALAASPASPALAGLU
6   ILEPROGLYTHRTRPLEUCYSARGASNGLY
7   METGLUGLYTHRLEUILEGLUGLYASPLEU
8   PROGLUPROLYSLYSVALLYSPROPROARG

Related Database Links:

BMRB 19149
PDB 2M4V 2M6P 4X8K
DBJ BAH26349 BAL66056 BAN31760 BAQ06108 GAA45755
EMBL CAB11301 CAC30389 CAL72057 CAR71533 CCC27135
GB AAK46389 AAS04115 ABK68564 ABQ73825 ABR06418
REF NP_216566 NP_302017 NP_855726 WP_003410601 WP_003872122
SP P9WHJ4 P9WHJ5
AlphaFold P9WHJ4 P9WHJ5

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