BMRB Entry 19025

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19025

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Title:
Solid-state Chemical Shift Assignments for CAP-Gly Domain of Mammalian at 19.9 T
Deposition date:
2013-02-11
Original release date:
2013-05-30
Authors:
Yan, Si; Hou, Guangjin; Schwieters, Charles; Ahmed, Shubbir; Williams, John; Polenova, Tatyana
Citation:

Citation: Yan, Si; Hou, Guangjin; Schwieters, Charles; Ahmed, Shubbir; Williams, John; Polenova, Tatyana. "Three-Dimensional Structure of CAP-Gly Domain of Mammalian Dynactin Determined by Magic Angle Spinning NMR Spectroscopy: Conformational Plasticity and Interactions with End-Binding Protein EB1."  J. Mol. Biol. 425, 4249-4266 (2013).
PubMed: 23648839

Assembly members:

Assembly members:
CAP-Gly, polymer, 89 residues, 9530 Da.

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28b-His6-SMT3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CAP-Gly: STEASARPLRVGSRVEVIGK GHRGTVAYVGATLFATGKWV GVILDEAKGKNDGTVQGRKY FTCDEGHGIFVRQSQIQVFE DGADTTSPE

Data sets:
Data typeCount
13C chemical shifts542
15N chemical shifts142

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1CAP-Gly1

Entities:

Entity 1, CAP-Gly 89 residues - 9530 Da.

The sequence starts from S19, and ends at E107. Residues S19-P26 and E98-E107 are not assigned due to the dynamics of the termini.

1   SERTHRGLUALASERALAARGPROLEUARG
2   VALGLYSERARGVALGLUVALILEGLYLYS
3   GLYHISARGGLYTHRVALALATYRVALGLY
4   ALATHRLEUPHEALATHRGLYLYSTRPVAL
5   GLYVALILELEUASPGLUALALYSGLYLYS
6   ASNASPGLYTHRVALGLNGLYARGLYSTYR
7   PHETHRCYSASPGLUGLYHISGLYILEPHE
8   VALARGGLNSERGLNILEGLNVALPHEGLU
9   ASPGLYALAASPTHRTHRSERPROGLU

Related Database Links:

BMRB 17937 17938 19031 25005
PDB 1TXQ 2HKN 2HKQ 2HL3 2HL5 2HQH 2M02 2MPX 3E2U 3TQ7
DBJ BAE34241 BAE37079 BAE42418 BAE42912 BAE87998
EMBL CAA44091 CAA67333 CAE45882 CAH10572 CAH10575
GB AAB57773 AAD03694 AAD55811 AAH66061 AAI42510
REF NP_001092404 NP_001127253 NP_001128512 NP_001177765 NP_001177766
SP O08788 P28023 Q14203
TPG DAA24857
AlphaFold O08788 P28023 Q14203