BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19026

Title: Analysis of the structural and molecular basis of voltage-sensitive sodium channel inhibition by the spider toxin, Huwentoxin-IV ( -TRTX-Hh2a).   PubMed: 23760503

Deposition date: 2013-02-11 Original release date: 2013-06-17

Authors: Gibbs, Al; Flinspach, Mack

Citation: Minassian, Natali; Gibbs, Alan; Shih, Amy; Liu, Yi; Neff, Robert; Sutton, Steven; Mirzadegan, Tara; Connor, Judith; Fellows, Ross; Husovsky, Matthew; Nelson, Serena; Hunter, Michael; Flinspach, Mack; Wickenden, Alan. "Analysis of the Structural and Molecular Basis of Voltage-sensitive Sodium Channel Inhibition by the Spider Toxin Huwentoxin-IV (-TRTX-Hh2a)."  J. Biol. Chem. 288, 22707-22720 (2013).

Assembly members:
HWTX-IV, polymer, 35 residues, 4120.876 Da.

Natural source:   Common Name: Chinese earth tiger   Taxonomy ID: 29017   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Ornithoctonus huwena

Experimental source:   Production method: recombinant technology   Host organism: Homo Sapien   Vector: pcDNA

Entity Sequences (FASTA):
HWTX-IV: ECLEIFKACNPSNDQCCKSS KLVCSRKTRWCKYQI

Data sets:
Data typeCount
1H chemical shifts194

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HWTX-IV1

Entities:

Entity 1, HWTX-IV 35 residues - 4120.876 Da.

1   GLUCYSLEUGLUILEPHELYSALACYSASN
2   PROSERASNASPGLNCYSCYSLYSSERSER
3   LYSLEUVALCYSSERARGLYSTHRARGTRP
4   CYSLYSTYRGLNILE

Samples:

sample_1: HWTX-IV 2 mM; sodium phosphate 20 mM; sodium azide 150 uM; EDTA 100 uM; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 950 MHz

Related Database Links:

BMRB 19030 19032
PDB
GB AAP33074 ABY77744 ABY77745 ABY77746
SP P83303
AlphaFold P83303