BMRB Entry 19053

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PDB ID: 2m5g
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19053
MolProbity Validation Chart

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Title:
Solution structure of FimA wt
Deposition date:
2013-02-24
Original release date:
2013-11-11
Authors:
Walczak, Michal; Puorger, Chasper; Glockshuber, Rudi; Wider, Gerhard
Citation:

Citation: Walczak, Michal; Puorger, Chasper; Glockshuber, Rudi; Wider, Gerhard. "Intramolecular Donor Strand Complementation in the E. coli Type 1 Pilus Subunit FimA Explains the Existence of FimA Monomers As Off-Pathway Products of Pilus Assembly That Inhibit Host Cell Apoptosis."  J. Mol. Biol. 426, 542-549 (2014).
PubMed: 24184277

Assembly members:

Assembly members:
FimA_wt, polymer, 159 residues, 15835.380 Da.

Natural source:

Natural source:   Common Name: Enterobacteria   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FimA_wt: AATTVNGGTVHFKGEVVNAA CAVDAGSVDQTVQLGQVRTA SLAQEGATSSAVGFNIQLND CDTNVASKAAVAFLGTAIDA GHTNVLALQSSAAGSATNVG VQILDRTGAALTLDGATFSS ETTLNNGTNTIPFQARYFAT GAATPGAANADATFKVQYQ

Data sets:
Data typeCount
1H chemical shifts668

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1FimA wt1

Entities:

Entity 1, FimA wt 159 residues - 15835.380 Da.

1   ALAALATHRTHRVALASNGLYGLYTHRVAL
2   HISPHELYSGLYGLUVALVALASNALAALA
3   CYSALAVALASPALAGLYSERVALASPGLN
4   THRVALGLNLEUGLYGLNVALARGTHRALA
5   SERLEUALAGLNGLUGLYALATHRSERSER
6   ALAVALGLYPHEASNILEGLNLEUASNASP
7   CYSASPTHRASNVALALASERLYSALAALA
8   VALALAPHELEUGLYTHRALAILEASPALA
9   GLYHISTHRASNVALLEUALALEUGLNSER
10   SERALAALAGLYSERALATHRASNVALGLY
11   VALGLNILELEUASPARGTHRGLYALAALA
12   LEUTHRLEUASPGLYALATHRPHESERSER
13   GLUTHRTHRLEUASNASNGLYTHRASNTHR
14   ILEPROPHEGLNALAARGTYRPHEALATHR
15   GLYALAALATHRPROGLYALAALAASNALA
16   ASPALATHRPHELYSVALGLNTYRGLN

Related Database Links:

BMRB 25334
PDB 2M5G 2MX3 2N7H 3SQB 4DWH
DBJ BAE78307 BAJ46028
EMBL CAA25489 CAQ34662 CAR16032 CAS12169 CCJ47011
GB AAA24389 AAA97210 AAC77270 AAG35683 AAO84630
REF NP_418734 WP_000695553 WP_000695557 WP_000695559 WP_000695560
SP P04128
AlphaFold P04128