BMRB Entry 19054

Name: NMR structures of human apoptotic protein tBid in LPPG micelle
Published: 2013-11-11

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PDB ID: 2m5i
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19054
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structures of human apoptotic protein tBid in LPPG micelle

Deposition date:

2013-02-25

Original release date:

2013-11-11

Authors:

Wang, Yu; Suzuki, Motoshi; Tjandra, Nico

Citation:

Citation: Wang, Yu; Tjandra, Nico. "Structural insights of tBid, the caspase-8-activated Bid, and its BH3 domain." J. Biol. Chem. 288, 35840-35851 (2013).
PubMed: 24158446

Assembly members:

Assembly members:
entity, polymer, 135 residues, 15243.369 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GNRSSHSRLGRIEADSESQE DIIRNIARHLAQVGDSMDRS IPPGLVNGLALQLRNTSRSE EDRNRDLATALEQLLQAYPR DMEKEKTMLVLALLLAKKVA SHTPSLLRDVFHTTVNFINQ NLRTYVRSLARNGMD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	564
15N chemical shifts	125
1H chemical shifts	908

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	human apoptotic protein tBid	1

Entities:

Entity 1, human apoptotic protein tBid 135 residues - 15243.369 Da.

1

GLY

ASN

ARG

SER

HIS

SER

ARG

LEU

GLY

2

ARG

ILE

GLU

ALA

ASP

SER

GLU

SER

GLN

GLU

3

ASP

ILE

ARG

ASN

ILE

ALA

ARG

HIS

LEU

4

ALA

GLN

VAL

GLY

ASP

SER

MET

ASP

ARG

SER

5

ILE

PRO

GLY

LEU

VAL

ASN

GLY

LEU

ALA

6

LEU

GLN

LEU

ARG

ASN

THR

SER

ARG

SER

GLU

7

GLU

ASP

ARG

ASN

ARG

ASP

LEU

ALA

THR

ALA

8

LEU

GLU

GLN

LEU

GLN

ALA

TYR

PRO

ARG

9

ASP

MET

GLU

LYS

GLU

LYS

THR

MET

LEU

VAL

10

LEU

ALA

LEU

ALA

LYS

VAL

ALA

11

SER

HIS

THR

PRO

SER

LEU

ARG

ASP

VAL

12

PHE

HIS

THR

VAL

ASN

PHE

ILE

ASN

GLN

13

ASN

LEU

ARG

THR

TYR

VAL

ARG

SER

LEU

ALA

14

ARG

ASN

GLY

MET

ASP

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
4D HCHC-NOESY	sample_2	isotropic	sample_conditions_1
T1	sample_1	isotropic	sample_conditions_1
T2	sample_1	isotropic	sample_conditions_1

PDB	2BID 2M5I
DBJ	BAF79673 BAG10922 BAG36471 BAG52933
EMBL	CAG28531 CAG30275 CAH92211 CAK54378 CAK54677
GB	AAC34365 AAH09197 AAH22072 AAH33634 AAH36364
REF	NP_001126293 NP_001187 NP_001231496 NP_001231498 NP_001231499
SP	P55957
AlphaFold	P55957

BMRB Entry 19054

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

Related Database Links: