BMRB Entry 19196

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PDB ID: 2m7m
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19196
MolProbity Validation Chart

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Title:
N-terminal domain of (Y81F)-EhCaBP1 structure
Deposition date:
2013-04-26
Original release date:
2013-06-17
Authors:
Chary, Kandala; Rout, Ashok; Patel, Sunita; Bhattacharya, Alok
Citation:

Citation: Rout, Ashok; Patel, Sunita; Somlata, .; Shukla, Manish; Saraswathi, Deepa; Bhattacharya, Alok; Chary, Kandala. "Functional manipulation of a calcium-binding protein from Entamoeba histolytica guided by paramagnetic NMR."  J. Biol. Chem. 288, 23473-23487 (2013).
PubMed: 23782698

Assembly members:

Assembly members:
(Y81F)-EhCaBP1, polymer, 66 residues, 7380.329 Da.
EhCaBP1, polymer, 134 residues, 851.608 Da.

Natural source:

Natural source:   Common Name: Eukaryotes   Taxonomy ID: 5759   Superkingdom: Eukaryota   Kingdom: not available   Genus/species: Entamoeba histolytica

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET30a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
(Y81F)-EhCaBP1: MAEALFKEIDVNGDGAVSYE EVKAFVSKKRAIKNEQLLQL IFKSIDADGNGEIDQNEFAK FYGSIQ
EhCaBP1: MAEALFKEIDVNGDGAVSYE EVKAFVSKKRAIKNEQLLQL IFKSIDADGNGEIDQNEFAK FYGSIQGQDLSDDKIGLKVL FKLMDVDGDGKLTKEEVTSF FKKHGIEKVAEQVMKADANG DGYITLEEFLEFSL

Data sets:
Data typeCount
13C chemical shifts248
15N chemical shifts60
1H chemical shifts321

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1(Y81F)-EhCaBP11
2EhCaBP12

Entities:

Entity 1, (Y81F)-EhCaBP1 66 residues - 7380.329 Da.

1   METALAGLUALALEUPHELYSGLUILEASP
2   VALASNGLYASPGLYALAVALSERTYRGLU
3   GLUVALLYSALAPHEVALSERLYSLYSARG
4   ALAILELYSASNGLUGLNLEULEUGLNLEU
5   ILEPHELYSSERILEASPALAASPGLYASN
6   GLYGLUILEASPGLNASNGLUPHEALALYS
7   PHETYRGLYSERILEGLN

Entity 2, EhCaBP1 134 residues - 851.608 Da.

1   METALAGLUALALEUPHELYSGLUILEASP
2   VALASNGLYASPGLYALAVALSERTYRGLU
3   GLUVALLYSALAPHEVALSERLYSLYSARG
4   ALAILELYSASNGLUGLNLEULEUGLNLEU
5   ILEPHELYSSERILEASPALAASPGLYASN
6   GLYGLUILEASPGLNASNGLUPHEALALYS
7   PHETYRGLYSERILEGLNGLYGLNASPLEU
8   SERASPASPLYSILEGLYLEULYSVALLEU
9   PHELYSLEUMETASPVALASPGLYASPGLY
10   LYSLEUTHRLYSGLUGLUVALTHRSERPHE
11   PHELYSLYSHISGLYILEGLULYSVALALA
12   GLUGLNVALMETLYSALAASPALAASNGLY
13   ASPGLYTYRILETHRLEUGLUGLUPHELEU
14   GLUPHESERLEU

Related Database Links:

BMRB 19193 19197 4271
PDB 2M7K 2M7M 3LI6 1JFJ 1JFK 2M7N 2NXQ 3PX1 3QJK 3ULG
DBJ BAN39246
GB AAA29089 EAL48959 EKE39141 EMD43507 EMH75928
REF XP_008858522 XP_654345
SP P38505
AlphaFold P38505

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