BMRB Entry 19249

Title:
NMR structure of the PAI subdomain of Sleeping Beauty transposase
Deposition date:
2013-05-17
Original release date:
2013-12-16
Authors:
Eubanks, Claire; Schreifels, Jeff; Aronovich, Elena; Carlson, Daniel; Hacjkett, Perry; Nesmelova, Irina
Citation:

Citation: Carpentier, Claire; Schreifels, Jeffrey; Aronovich, Elena; Carlson, Daniel; Hackett, Perry; Nesmelova, Irina. "NMR structural analysis of Sleeping Beauty transposase binding to DNA."  Protein Sci. 23, 23-33 (2014).
PubMed: 24243759

Assembly members:

Assembly members:
entity, polymer, 57 residues, 6394.522 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a(+)

Entity Sequences (FASTA):

Data sets:
Data typeCount
15N chemical shifts54
1H chemical shifts322

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1PAI subdomain of Sleeping Beauty transposase1

Entities:

Entity 1, PAI subdomain of Sleeping Beauty transposase 57 residues - 6394.522 Da.

1   ALASERMETGLYLYSSERLYSGLUILESER
2   GLNASPLEUARGLYSLYSILEVALASPLEU
3   HISLYSSERGLYSERSERLEUGLYALAILE
4   SERLYSARGLEULYSVALPROARGSERSER
5   VALGLNTHRILEVALARGLYSTYRLYSHIS
6   HISGLYTHRTHRGLNHISHIS

Related Database Links:

PDB 2M8E
EMBL CDQ61086 CDQ84631
GB AFR53956

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks