BMRB Entry 19368

Name: Structure of Pex14 in complex with Pex5 LVxEF motif
Published: 2013-11-26

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PDB ID: 4bxu
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19368
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of Pex14 in complex with Pex5 LVxEF motif

Deposition date:

2013-07-16

Original release date:

2013-11-26

Authors:

Kooshapur, Hamed; Meyer, H.; Madl, T.; Sattler, M.

Citation:

Citation: Neuhaus, Alexander; Kooshapur, Hamed; Wolf, Janina; Meyer, N. Helge; Madl, Tobias; Saidowsky, Jurgen; Hambruch, Eva; Lazam, Anissa; Jung, Martin; Sattler, Michael; Schliebs, Wolfgang; Erdmann, Ralf. "A Novel Pex14 Protein-interacting Site of Human Pex5 Is Critical for Matrix Protein Import into Peroxisomes." J. Biol. Chem. 289, 437-448 (2014).
PubMed: 24235149

Assembly members:

Assembly members:
PEROXISOMAL_MEMBRANE_PROTEIN_PEX14, polymer, 69 residues, 7445.4517 Da.
PEROXISOMAL_TARGETING_SIGNAL_1_RECEPTOR, polymer, 15 residues, 1701.7162 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: n/a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PEROXISOMAL_MEMBRANE_PROTEIN_PEX14: GAMATPGSENVLPREPLIAT AVKFLQNSRVRQSPLATRRA FLKKKGLTDEEIDMAFQQSG TAADEPSSL
PEROXISOMAL_TARGETING_SIGNAL_1_RECEPTOR: ASEDELVAEFLQDQN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_1_2

Data type	Count
13C chemical shifts	415
15N chemical shifts	124
1H chemical shifts	847

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PEROXISOMAL MEMBRANE PROTEIN PEX14	1
2	PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR	2

Entities:

Entity 1, PEROXISOMAL MEMBRANE PROTEIN PEX14 69 residues - 7445.4517 Da.

1

GLY

ALA

MET

ALA

THR

PRO

GLY

SER

GLU

ASN

2

VAL

LEU

PRO

ARG

GLU

PRO

LEU

ILE

ALA

THR

3

ALA

VAL

LYS

PHE

LEU

GLN

ASN

SER

ARG

VAL

4

ARG

GLN

SER

PRO

LEU

ALA

THR

ARG

ALA

5

PHE

LEU

LYS

GLY

LEU

THR

ASP

GLU

6

GLU

ILE

ASP

MET

ALA

PHE

GLN

SER

GLY

7

THR

ALA

ASP

GLU

PRO

SER

LEU

Entity 2, PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR 15 residues - 1701.7162 Da.

1

ALA

SER

GLU

ASP

GLU

LEU

VAL

ALA

GLU

PHE

2

LEU

GLN

ASP

GLN

ASN

Samples:

sample_1: PEROXISOMAL MEMBRANE PROTEIN PEX14, [U-13C; U-15N], mM; PEROXISOMAL TARGETING SIGNAL 1 RECEPTOR, [U-13C; U-15N], mM

sample_conditions_1: ionic strength: 0.0 mM; pH: 6.500; pressure: 1 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
Experiment_1	sample_1	solution	sample_conditions_1

Software:

ARIA1.2_CNS vany, JP Linge, SI O Donoghue and M Nilges -

AutoDep v4.3 -

NMR spectrometers:

Bruker Avance 0 MHz

UNP	PEX14_HUMAN PEX5_HUMAN
PDB	2W84 2W85 3FF5 4BXU
DBJ	BAA36837 BAE00503 BAE88740 BAG35878 BAG51028
EMBL	CAG29317 CAG46880
GB	AAC39843 AAH06327 AAX36586 ABM83701 ABM86831
REF	NP_001271931 NP_004556 XP_001118790 XP_001162201 XP_002811556 XP_004052675
SP	O75381
AlphaFold	Q8WX51 O75381 Q96FN7