BMRB Entry 19411
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR19411
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Burmann, Bjoern; Wang, Congwei; Hiller, Sebastian. "Conformation and dynamics of the periplasmic membrane-protein-chaperone complexes OmpX-Skp and tOmpA-Skp" Nat. Struct. Mol. Biol. 20, 1265-1272 (2013).
PubMed: 24077225
Assembly members:
Escherichia_Coli_OmpX, polymer, 149 residues, Formula weight is not available
Escherichia_Coli_Skp, polymer, 234 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28B-OmpX
Entity Sequences (FASTA):
Escherichia_Coli_OmpX: MATSTVTGGYAQSDMQGVMN
KTNGFNLKYRYEQQNNPLGV
IGSFTYTEKDRTASSGDYNK
NQYYGITAGPAYRINDWASI
YGVVGVGYGKFQTTEYPTYK
HDTSDYGFSYGAGLQFNPME
NVALDFSYEQSRIRSVDVGT
WIAGVGYRF
Escherichia_Coli_Skp: MGSSHHHHHHSSGLVPRGSH
MADKIAIVNMGSLFQQVAQK
TGVSNTLENEFKGRASELQR
METDLQAKMKKLQSMKAGSD
RTKLEKDVMAQRQTFAQKAQ
AFEQDRARRSNEERGKLVTR
IQTAVKSVANSQDIDLVVDA
NAVAYNSSDVKDITADVLKQ
VKIYGVVGVGYGKFQQTENQ
GLNRTASNSDYGFSYGAGMQ
FNPIENVALDFSYEQSRIRN
VDVGTWIAGVGYRF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 236 |
| 15N chemical shifts | 129 |
| 1H chemical shifts | 129 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | OmpX | 1 |
| 2 | Skp, chain 1 | 2 |
| 3 | Skp, chain 2 | 2 |
| 4 | Skp, chain 3 | 2 |
Entities:
Entity 1, OmpX 149 residues - Formula weight is not available
native processed E. coli OmpX (rseidues 24 - 172) + initiator methionine
| 1 | MET | ALA | THR | SER | THR | VAL | THR | GLY | GLY | TYR | ||||
| 2 | ALA | GLN | SER | ASP | MET | GLN | GLY | VAL | MET | ASN | ||||
| 3 | LYS | THR | ASN | GLY | PHE | ASN | LEU | LYS | TYR | ARG | ||||
| 4 | TYR | GLU | GLN | GLN | ASN | ASN | PRO | LEU | GLY | VAL | ||||
| 5 | ILE | GLY | SER | PHE | THR | TYR | THR | GLU | LYS | ASP | ||||
| 6 | ARG | THR | ALA | SER | SER | GLY | ASP | TYR | ASN | LYS | ||||
| 7 | ASN | GLN | TYR | TYR | GLY | ILE | THR | ALA | GLY | PRO | ||||
| 8 | ALA | TYR | ARG | ILE | ASN | ASP | TRP | ALA | SER | ILE | ||||
| 9 | TYR | GLY | VAL | VAL | GLY | VAL | GLY | TYR | GLY | LYS | ||||
| 10 | PHE | GLN | THR | THR | GLU | TYR | PRO | THR | TYR | LYS | ||||
| 11 | HIS | ASP | THR | SER | ASP | TYR | GLY | PHE | SER | TYR | ||||
| 12 | GLY | ALA | GLY | LEU | GLN | PHE | ASN | PRO | MET | GLU | ||||
| 13 | ASN | VAL | ALA | LEU | ASP | PHE | SER | TYR | GLU | GLN | ||||
| 14 | SER | ARG | ILE | ARG | SER | VAL | ASP | VAL | GLY | THR | ||||
| 15 | TRP | ILE | ALA | GLY | VAL | GLY | TYR | ARG | PHE |
Entity 2, Skp, chain 1 234 residues - Formula weight is not available
native processed E. coli SKP (residues 21 - 161) hexahistidin-tag and Thrombin cleavage site (coming from pET28b)
| 1 | MET | GLY | SER | SER | HIS | HIS | HIS | HIS | HIS | HIS | ||||
| 2 | SER | SER | GLY | LEU | VAL | PRO | ARG | GLY | SER | HIS | ||||
| 3 | MET | ALA | ASP | LYS | ILE | ALA | ILE | VAL | ASN | MET | ||||
| 4 | GLY | SER | LEU | PHE | GLN | GLN | VAL | ALA | GLN | LYS | ||||
| 5 | THR | GLY | VAL | SER | ASN | THR | LEU | GLU | ASN | GLU | ||||
| 6 | PHE | LYS | GLY | ARG | ALA | SER | GLU | LEU | GLN | ARG | ||||
| 7 | MET | GLU | THR | ASP | LEU | GLN | ALA | LYS | MET | LYS | ||||
| 8 | LYS | LEU | GLN | SER | MET | LYS | ALA | GLY | SER | ASP | ||||
| 9 | ARG | THR | LYS | LEU | GLU | LYS | ASP | VAL | MET | ALA | ||||
| 10 | GLN | ARG | GLN | THR | PHE | ALA | GLN | LYS | ALA | GLN | ||||
| 11 | ALA | PHE | GLU | GLN | ASP | ARG | ALA | ARG | ARG | SER | ||||
| 12 | ASN | GLU | GLU | ARG | GLY | LYS | LEU | VAL | THR | ARG | ||||
| 13 | ILE | GLN | THR | ALA | VAL | LYS | SER | VAL | ALA | ASN | ||||
| 14 | SER | GLN | ASP | ILE | ASP | LEU | VAL | VAL | ASP | ALA | ||||
| 15 | ASN | ALA | VAL | ALA | TYR | ASN | SER | SER | ASP | VAL | ||||
| 16 | LYS | ASP | ILE | THR | ALA | ASP | VAL | LEU | LYS | GLN | ||||
| 17 | VAL | LYS | ILE | TYR | GLY | VAL | VAL | GLY | VAL | GLY | ||||
| 18 | TYR | GLY | LYS | PHE | GLN | GLN | THR | GLU | ASN | GLN | ||||
| 19 | GLY | LEU | ASN | ARG | THR | ALA | SER | ASN | SER | ASP | ||||
| 20 | TYR | GLY | PHE | SER | TYR | GLY | ALA | GLY | MET | GLN | ||||
| 21 | PHE | ASN | PRO | ILE | GLU | ASN | VAL | ALA | LEU | ASP | ||||
| 22 | PHE | SER | TYR | GLU | GLN | SER | ARG | ILE | ARG | ASN | ||||
| 23 | VAL | ASP | VAL | GLY | THR | TRP | ILE | ALA | GLY | VAL | ||||
| 24 | GLY | TYR | ARG | PHE |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks