BMRB Entry 19422
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PDB ID: 2mc0
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19422
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Habazettl, Judith; Allan, Martin; Jensen, Pernille; Sass, J rgen; Grzesiek, Stephan. "Structural Basis of a Thiopeptide Antibiotic Multidrug Resistance System from Streptomyces lividans" .
Assembly members:
TipAS, polymer, 144 residues, 16453.025 Da.
nosiheptide, polymer, 14 residues, 1050.172 Da.
Natural source: Common Name: Streptomyces lividans Taxonomy ID: 1916 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptomyces lividans
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDS8
Entity Sequences (FASTA):
TipAS: MGINLTPEEKFEVFGDFDPD
QYEEEVRERWGNTDAYRQSK
EKTASYTKEDWQRIQDEADE
LTRRFVALMDAGEPADSEGA
MDAAEDHRQGIARNHYDCGY
EMHTCLGEMYVSDERFTRNI
DAAKPGLAAYMRDAILANAV
RHTP
nosiheptide: SXTXXXXCXXXXXX
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 600 |
| 15N chemical shifts | 153 |
| 1H chemical shifts | 983 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TipAS | 1 |
| 2 | nosiheptide | 2 |
Entities:
Entity 1, TipAS 144 residues - 16453.025 Da.
TipAS is the short isoform of TipA. It is the C terminal domain from residue M110 to P253.
| 1 | MET | GLY | ILE | ASN | LEU | THR | PRO | GLU | GLU | LYS | ||||
| 2 | PHE | GLU | VAL | PHE | GLY | ASP | PHE | ASP | PRO | ASP | ||||
| 3 | GLN | TYR | GLU | GLU | GLU | VAL | ARG | GLU | ARG | TRP | ||||
| 4 | GLY | ASN | THR | ASP | ALA | TYR | ARG | GLN | SER | LYS | ||||
| 5 | GLU | LYS | THR | ALA | SER | TYR | THR | LYS | GLU | ASP | ||||
| 6 | TRP | GLN | ARG | ILE | GLN | ASP | GLU | ALA | ASP | GLU | ||||
| 7 | LEU | THR | ARG | ARG | PHE | VAL | ALA | LEU | MET | ASP | ||||
| 8 | ALA | GLY | GLU | PRO | ALA | ASP | SER | GLU | GLY | ALA | ||||
| 9 | MET | ASP | ALA | ALA | GLU | ASP | HIS | ARG | GLN | GLY | ||||
| 10 | ILE | ALA | ARG | ASN | HIS | TYR | ASP | CYS | GLY | TYR | ||||
| 11 | GLU | MET | HIS | THR | CYS | LEU | GLY | GLU | MET | TYR | ||||
| 12 | VAL | SER | ASP | GLU | ARG | PHE | THR | ARG | ASN | ILE | ||||
| 13 | ASP | ALA | ALA | LYS | PRO | GLY | LEU | ALA | ALA | TYR | ||||
| 14 | MET | ARG | ASP | ALA | ILE | LEU | ALA | ASN | ALA | VAL | ||||
| 15 | ARG | HIS | THR | PRO |
Entity 2, nosiheptide 14 residues - 1050.172 Da.
| 1 | SER | BB9 | THR | DBU | BB9 | 3GL | BB9 | CYS | BB9 | MH6 | ||||
| 2 | BB9 | DHA | NH2 | NO1 |
Related Database Links:
| BMRB | 19421 |
| PDB | 1NY9 2MBZ 2MC0 |
| DBJ | BAD11210 BAD11216 BAD11222 BAD11228 BAD11234 |
| EMBL | CAB42766 |
| GB | AAB27737 AAC13653 AIJ15084 AIV35032 EFD68501 |
| REF | NP_627619 WP_003975420 WP_007452041 WP_052836374 |
| SP | P0A4T8 P0A4T9 |
| AlphaFold | P0A4T8 P0A4T9 |
Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone
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