BMRB Entry 19516
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PDB ID: 2mv7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19516
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Kuntimaddi, Aravinda; Bushweller, John. "Solution NMR structure of Dot1L in complex with AF9 (Dot1L-AF9)." .
Assembly members:
Protein_AF-9, polymer, 69 residues, 8147.291 Da.
Dot1L_Histone-lysine_N-methyltransferase, polymer, 25 residues, 2667.140 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETduet-1
Entity Sequences (FASTA):
Protein_AF-9: DKAYLDELVELHRRLMTLRE
RHILQQIVNLIEETGHFHIT
NTTFDFDLCSLDKTTVRKLQ
SYLETSGTS
Dot1L_Histone-lysine_N-methyltransferase: TNKLPVSIPLASVVLPSRAE
RARST
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 414 |
| 15N chemical shifts | 89 |
| 1H chemical shifts | 638 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AF9 protein | 1 |
| 2 | Dot1L Histone lysine N-methyltransferase | 2 |
Entities:
Entity 1, AF9 protein 69 residues - 8147.291 Da.
| 1 | ASP | LYS | ALA | TYR | LEU | ASP | GLU | LEU | VAL | GLU | ||||
| 2 | LEU | HIS | ARG | ARG | LEU | MET | THR | LEU | ARG | GLU | ||||
| 3 | ARG | HIS | ILE | LEU | GLN | GLN | ILE | VAL | ASN | LEU | ||||
| 4 | ILE | GLU | GLU | THR | GLY | HIS | PHE | HIS | ILE | THR | ||||
| 5 | ASN | THR | THR | PHE | ASP | PHE | ASP | LEU | CYS | SER | ||||
| 6 | LEU | ASP | LYS | THR | THR | VAL | ARG | LYS | LEU | GLN | ||||
| 7 | SER | TYR | LEU | GLU | THR | SER | GLY | THR | SER |
Entity 2, Dot1L Histone lysine N-methyltransferase 25 residues - 2667.140 Da.
| 1 | THR | ASN | LYS | LEU | PRO | VAL | SER | ILE | PRO | LEU | ||||
| 2 | ALA | SER | VAL | VAL | LEU | PRO | SER | ARG | ALA | GLU | ||||
| 3 | ARG | ALA | ARG | SER | THR |
Related Database Links:
| BMRB | 18094 |
| PDB | 2LM0 2MV7 |
| DBJ | BAA04090 BAB31731 BAE27603 BAG35760 BAG72836 |
| EMBL | CAA06960 CDQ62412 CDQ68675 |
| GB | AAA58361 AAH21420 AAH36089 AAI29090 AAK28536 |
| REF | NP_001179478 NP_001273087 NP_001273620 NP_004520 NP_081602 |
| SP | A2AM29 P42568 |
| TPG | DAA26970 |
| AlphaFold | A2AM29 P42568 Q96JL1 |
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