BMRB Entry 19550

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PDB ID: 2mfi
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19550
MolProbity Validation Chart

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Title:
Domain 1 of E. coli ribosomal protein S1
Deposition date:
2013-10-11
Original release date:
2014-04-14
Authors:
GIRAUD, Pierre; CRECHET, Jean-Bernard; BONTEMS, Francois; UZAN, Marc; SIZUN, Christina
Citation:

Citation: Giraud, Pierre; Crechet, Jean-Bernard; Uzan, Marc; Bontems, Francois; Sizun, Christina. "Resonance assignment of the ribosome binding domain of E. coli ribosomal protein S1."  Biomol. NMR Assignments ., .-. (2014).
PubMed: 24682851

Assembly members:

Assembly members:
S1F1, polymer, 96 residues, 10408.6085 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET151:D-TOPO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
S1F1: GIDPFTESLKEIETRPGSIV RGVVVAIDKDVVLVDAGLKS ESAIPAEQFKNAQGELEIQV GDEVDVALDAVEDGFGETLL SREKAKRHEAWITLEK

Data sets:
Data typeCount
13C chemical shifts389
15N chemical shifts95
1H chemical shifts659

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1S1F11

Entities:

Entity 1, S1F1 96 residues - 10408.6085 Da.

The six first residues are generated by TOPO cloning and TEV cleavage.

1   GLYILEASPPROPHETHRGLUSERLEULYS
2   GLUILEGLUTHRARGPROGLYSERILEVAL
3   ARGGLYVALVALVALALAILEASPLYSASP
4   VALVALLEUVALASPALAGLYLEULYSSER
5   GLUSERALAILEPROALAGLUGLNPHELYS
6   ASNALAGLNGLYGLULEUGLUILEGLNVAL
7   GLYASPGLUVALASPVALALALEUASPALA
8   VALGLUASPGLYPHEGLYGLUTHRLEULEU
9   SERARGGLULYSALALYSARGHISGLUALA
10   TRPILETHRLEUGLULYS

Related Database Links:

UniProt P0AG67
BMRB 19554
PDB 2MFI 2MFL 4Q7J 4R71 4TOI
DBJ BAA35655 BAB34417 BAG76494 BAH62634 BAI24353
EMBL CAA23630 CAA23644 CAD05381 CAP75381 CAQ31439
GB AAC73997 AAG55396 AAL19915 AAN42537 AAN79519
PIR AC0614
PRF 0804233A
REF NP_309021 NP_415431 NP_455468 NP_459956 NP_706830
SP P0AG67 P0AG68 P0AG69 P0AG70
AlphaFold P77352 P0AG67 P0AG68 P0AG69 P0AG70

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