BMRB Entry 19646

Title:
Solution structure of the major factor VIII binding region on von Willebrand factor
Deposition date:
2013-12-02
Original release date:
2014-04-09
Authors:
Shiltagh, Nuha; Kirkpatrick, John; Cabrita, Lisa; McKinnon, Tom; Thalassinos, Konstantinos; Tuddenham, Edward; Hansen, Flemming
Citation:

Citation: Shiltagh, Nuha; Kirkpatrick, John; Cabrita, Lisa; McKinnon, Tom; Thalassinos, Konstantinos; Tuddenham, Edward; Hansen, D. Flemming. "Solution structure of the major factor VIII binding region on von Willebrand factor."  Blood ., .-. (2014).
PubMed: 24700780

Assembly members:

Assembly members:
VWF_TIL-E, polymer, 103 residues, 11351.283 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET32b+

Data sets:
Data typeCount
13C chemical shifts287
15N chemical shifts85
1H chemical shifts587

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1major factor VIII binding region on von Willebrand factor1

Entities:

Entity 1, major factor VIII binding region on von Willebrand factor 103 residues - 11351.283 Da.

Residues 1-4 (GAMG) are cloning artefacts. The VWF sequence proper begins at residue 5, and represents residues 766-864 in full-length VWF.

1   GLYALAMETGLYSERCYSARGPROPROMET
2   VALLYSLEUVALCYSPROALAASPASNLEU
3   ARGALAGLUGLYLEUGLUCYSTHRLYSTHR
4   CYSGLNASNTYRASPLEUGLUCYSMETSER
5   METGLYCYSVALSERGLYCYSLEUCYSPRO
6   PROGLYMETVALARGHISGLUASNARGCYS
7   VALALALEUGLUARGCYSPROCYSPHEHIS
8   GLNGLYLYSGLUTYRALAPROGLYGLUTHR
9   VALLYSILEGLYCYSASNTHRCYSVALCYS
10   ARGASPARGLYSTRPASNCYSTHRASPHIS
11   VALCYSASP

Related Database Links:

PDB 2MHP 2MHQ
GB AAB59512

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks