BMRB Entry 19694

Name: Structure of FHL2 LIM adaptor and its Interaction with Ski
Published: 2014-01-13

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PDB ID: 2miu
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19694
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of FHL2 LIM adaptor and its Interaction with Ski

Deposition date:

2013-12-20

Original release date:

2014-01-13

Authors:

Yang, Yanwu; Sun, Yaqin; Medrano, Estela; Yian, Xiao-Li; Weiss, Michael

Citation:

Citation: Yang, Yanwu; Sun, Yaqin; Medrano, Estela; Yian, Xiao-Li; Weiss, Michael. "Structure of FHL2 LIM adaptor and its Interaction with Ski" J. Biol. Chem. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 98 residues, 11485.008 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-5X-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MTERFDCHHCNESLFGKKYI LREESPYCVVCFETLFANTC EECGKPIGCDCKDLSYKDRH WHEACFHCSQCRNSLVDKPF AAKEDQLLCTDCYSNEYS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	601
13C chemical shifts	303
15N chemical shifts	101

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FHL2 LIM adaptor	1
2	ZINC ION_1	2
3	ZINC ION_2	2
4	ZINC ION_3	2

Entities:

Entity 1, FHL2 LIM adaptor 98 residues - 11485.008 Da.

1

MET

THR

GLU

ARG

PHE

ASP

CYS

HIS

CYS

2

ASN

GLU

SER

LEU

PHE

GLY

LYS

TYR

ILE

3

LEU

ARG

GLU

SER

PRO

TYR

CYS

VAL

4

CYS

PHE

GLU

THR

LEU

PHE

ALA

ASN

THR

CYS

5

GLU

CYS

GLY

LYS

PRO

ILE

GLY

CYS

ASP

6

CYS

LYS

ASP

LEU

SER

TYR

LYS

ASP

ARG

HIS

7

TRP

HIS

GLU

ALA

CYS

PHE

HIS

CYS

SER

GLN

8

CYS

ARG

ASN

SER

LEU

VAL

ASP

LYS

PRO

PHE

9

ALA

LYS

GLU

ASP

GLN

LEU

CYS

THR

10

ASP

CYS

TYR

SER

ASN

GLU

TYR

SER

Entity 2, ZINC ION_1 - 65.409 Da.

1

ZN

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

PDB	2MIU
DBJ	BAD69710
GB	AAY14896
REF	XP_011509101 XP_011509102

BMRB Entry 19694

Additional metadata:

Assembly:

Entities:

Samples:

Experiments:

Software:

NMR spectrometers:

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