BMRB Entry 19709

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PDB ID: 2mj7
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19709
MolProbity Validation Chart

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Title:
Solution NMR structure of beta-adaptin appendage domain of human adaptor protein complex 4 subunit beta, Northeast Structural Genomics Consortium (NESG) Target HR8998C
Deposition date:
2013-12-26
Original release date:
2014-01-23
Authors:
Eletsky, Alexander; Rotshteyn, Daniel; Pederson, Kari; Shastry, Ritu; Maglaqui, Melissa; Janjua, Haleema; Xiao, Rong; Everett, John; Montelione, Gaetano; Prestegard, James; Szyperski, Thomas
Citation:

Citation: Eletsky, Alexander; Rotshteyn, Daniel; Pederson, Kari; Shastry, Ritu; Maglaqui, Melissa; Janjua, Haleema; Xiao, Rong; Everett, John; Montelione, Gaetano; Prestegard, James; Szyperski, Thomas. "Solution NMR structure of beta-adaptin appendage domain of human adaptor protein complex 4 subunit beta, Northeast Structural Genomics Consortium (NESG) Target HR8998C"  To be published ., .-..

Assembly members:

Assembly members:
HR8998C, polymer, 141 residues, 16004.294 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15_NESG

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HR8998C: MGHHHHHHSHMQELPDSGAL MLVPNRQLTADYFEKTWLSL KVAHQQVLPWRGEFHPDTLQ MALQVVNIQTIAMSRAGSRP WKAYLSAQDDTGCLFLTELL LEPGNSEMQISVKQNEARTE TLNSFISVLETVIGTIEEIK S

Data sets:
Data typeCount
13C chemical shifts584
15N chemical shifts147
1H chemical shifts970

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1HR8998C1

Entities:

Entity 1, HR8998C 141 residues - 16004.294 Da.

Residues 12-141 correspond to fragment 610-739 of the native sequence. Residues 1-11 represent a non-native purification tag.

1   METGLYHISHISHISHISHISHISSERHIS
2   METGLNGLULEUPROASPSERGLYALALEU
3   METLEUVALPROASNARGGLNLEUTHRALA
4   ASPTYRPHEGLULYSTHRTRPLEUSERLEU
5   LYSVALALAHISGLNGLNVALLEUPROTRP
6   ARGGLYGLUPHEHISPROASPTHRLEUGLN
7   METALALEUGLNVALVALASNILEGLNTHR
8   ILEALAMETSERARGALAGLYSERARGPRO
9   TRPLYSALATYRLEUSERALAGLNASPASP
10   THRGLYCYSLEUPHELEUTHRGLULEULEU
11   LEUGLUPROGLYASNSERGLUMETGLNILE
12   SERVALLYSGLNASNGLUALAARGTHRGLU
13   THRLEUASNSERPHEILESERVALLEUGLU
14   THRVALILEGLYTHRILEGLUGLUILELYS
15   SER

Related Database Links:

UNP Q9Y6B7
PDB 2MJ7
GB EHH15072 EHH50179
REF XP_001110491 XP_001110876 XP_003892466 XP_005542316 XP_005542317
AlphaFold Q96CL6

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