BMRB Entry 19735

Assembly:

Entities:

Entity 1, TIA-1 RRM2,3 monomer 185 residues - 20568.250 Da.

Residues 89-92 (1-4) represent a tag from used construct after TEV cleavage, and residues 93-274 (5-186) are derived from the protein sequence of TIA-1.

Samples:

sample_1: TIA-1 RRM2,3, [U-99% 13C; U-99% 15N], 0.1-0.7 mM; sodium phosphate 10 mM; sodium chloride 50 mM; DTT 10 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_2: TIA-1 RRM2,3, [U-99% 15N], 0.1-0.55 mM; sodium phosphate 10 mM; sodium chloride 50 mM; DTT 10 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_3: TIA-1 RRM2,3, [U-10% 13C], 0.1-0.5 mM; sodium phosphate 10 mM; sodium chloride 50 mM; DTT 10 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN, Bruker Biospin - collection, data analysis, processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking, processing

X-PLOR_NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement

SPARKY, Goddard - chemical shift assignment, data analysis, peak picking

TALOS, Cornilescu, Delaglio and Bax - chemical shift calculation, data analysis

NMR spectrometers:

• Bruker Avance 600 MHz
• Bruker Avance 750 MHz
• Bruker Avance 800 MHz
• Bruker Avance 900 MHz