BMRB Entry 19836

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PDB ID: 2mly
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR19836
MolProbity Validation Chart

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Title:
NMR structure of E. coli Trigger Factor in complex with unfolded PhoA1-150
Deposition date:
2014-03-05
Original release date:
2014-05-20
Authors:
Saio, Tomohide; Guan, Xiao; Rossi, Paolo; Economou, Anastassios; Kalodimos, Charalampos
Citation:

Citation: Saio, Tomohide; Guan, Xiao; Rossi, Paolo; Economou, Anastassios; Kalodimos, Charalampos. "Structural basis for protein antiaggregation activity of the trigger factor chaperone"  Science 344, 1250494-1250494 (2014).
PubMed: 24812405

Assembly members:

Assembly members:
entity_1, polymer, 151 residues, 16862.254 Da.
entity_2, polymer, 443 residues, 48256.020 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 469008   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: HMKQSTIALALLPLLFTPVT KARTPEMPVLENRAAQGDIT APGGARRLTGDQTAALRDSL SDKPAKNIILLIGDGMGDSE ITAARNYAEGAGGFFKGIDA LPLTGQYTHYALNKKTGKPD YVTDSAASATAWSTGVKTYN GALGVDIHEKD
entity_2: MNHKVHHHHHHMQVSVETTQ GLGRRVTITIAADSIETAVK SELVNVAKKVRIDGFRKGKV PMNIVAQRYGASVRQDVLGD LMSRNFIDAIIKEKINPAGA PTYVPGEYKLGEDFTYSVEF EVYPEVELQGLEAIEVEKPI VEVTDADVDGMLDTLRKQQA TWKEKDGAVEAEDRVTIDFT GSVDGEEFEGGKASDFVLAM GQGRMIPGFEDGIKGHKAGE EFTIDVTFPEEYHAENLKGK AAKFAINLKKVEERELPELT AEFIKRFGVEDGSVEGLRAE VRKNMERELKSAIRNRVKSQ AIEGLVKANDIDVPAALIDS EIDVLRRQAAQRFGGNEKQA LELPRELFEEQAKRRVVVGL LLGEVIRTNELKADEERVKG LIEEMASAYEDPKEVIEFYS KNKELMDNMRNVALEEQAVE AVLAKAKVTEKETTFNELMN QQA

Data typeCount
13C chemical shifts565
15N chemical shifts479
1H chemical shifts1638

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 151 residues - 16862.254 Da.

1   HISMETLYSGLNSERTHRILEALALEUALA
2   LEULEUPROLEULEUPHETHRPROVALTHR
3   LYSALAARGTHRPROGLUMETPROVALLEU
4   GLUASNARGALAALAGLNGLYASPILETHR
5   ALAPROGLYGLYALAARGARGLEUTHRGLY
6   ASPGLNTHRALAALALEUARGASPSERLEU
7   SERASPLYSPROALALYSASNILEILELEU
8   LEUILEGLYASPGLYMETGLYASPSERGLU
9   ILETHRALAALAARGASNTYRALAGLUGLY
10   ALAGLYGLYPHEPHELYSGLYILEASPALA
11   LEUPROLEUTHRGLYGLNTYRTHRHISTYR
12   ALALEUASNLYSLYSTHRGLYLYSPROASP
13   TYRVALTHRASPSERALAALASERALATHR
14   ALATRPSERTHRGLYVALLYSTHRTYRASN
15   GLYALALEUGLYVALASPILEHISGLULYS
16   ASP

Entity 2, entity_2 443 residues - 48256.020 Da.

1   METASNHISLYSVALHISHISHISHISHIS
2   HISMETGLNVALSERVALGLUTHRTHRGLN
3   GLYLEUGLYARGARGVALTHRILETHRILE
4   ALAALAASPSERILEGLUTHRALAVALLYS
5   SERGLULEUVALASNVALALALYSLYSVAL
6   ARGILEASPGLYPHEARGLYSGLYLYSVAL
7   PROMETASNILEVALALAGLNARGTYRGLY
8   ALASERVALARGGLNASPVALLEUGLYASP
9   LEUMETSERARGASNPHEILEASPALAILE
10   ILELYSGLULYSILEASNPROALAGLYALA
11   PROTHRTYRVALPROGLYGLUTYRLYSLEU
12   GLYGLUASPPHETHRTYRSERVALGLUPHE
13   GLUVALTYRPROGLUVALGLULEUGLNGLY
14   LEUGLUALAILEGLUVALGLULYSPROILE
15   VALGLUVALTHRASPALAASPVALASPGLY
16   METLEUASPTHRLEUARGLYSGLNGLNALA
17   THRTRPLYSGLULYSASPGLYALAVALGLU
18   ALAGLUASPARGVALTHRILEASPPHETHR
19   GLYSERVALASPGLYGLUGLUPHEGLUGLY
20   GLYLYSALASERASPPHEVALLEUALAMET
21   GLYGLNGLYARGMETILEPROGLYPHEGLU
22   ASPGLYILELYSGLYHISLYSALAGLYGLU
23   GLUPHETHRILEASPVALTHRPHEPROGLU
24   GLUTYRHISALAGLUASNLEULYSGLYLYS
25   ALAALALYSPHEALAILEASNLEULYSLYS
26   VALGLUGLUARGGLULEUPROGLULEUTHR
27   ALAGLUPHEILELYSARGPHEGLYVALGLU
28   ASPGLYSERVALGLUGLYLEUARGALAGLU
29   VALARGLYSASNMETGLUARGGLULEULYS
30   SERALAILEARGASNARGVALLYSSERGLN
31   ALAILEGLUGLYLEUVALLYSALAASNASP
32   ILEASPVALPROALAALALEUILEASPSER
33   GLUILEASPVALLEUARGARGGLNALAALA
34   GLNARGPHEGLYGLYASNGLULYSGLNALA
35   LEUGLULEUPROARGGLULEUPHEGLUGLU
36   GLNALALYSARGARGVALVALVALGLYLEU
37   LEULEUGLYGLUVALILEARGTHRASNGLU
38   LEULYSALAASPGLUGLUARGVALLYSGLY
39   LEUILEGLUGLUMETALASERALATYRGLU
40   ASPPROLYSGLUVALILEGLUPHETYRSER
41   LYSASNLYSGLULEUMETASPASNMETARG
42   ASNVALALALEUGLUGLUGLNALAVALGLU
43   ALAVALLEUALALYSALALYSVALTHRGLU
44   LYSGLUTHRTHRPHEASNGLULEUMETASN
45   GLNGLNALA

Related Database Links:

PDB 1AJA 1AJB 1AJC 1AJD 1ALH 1ALI 1ALJ 1ALK 1ANI 1ANJ 1B8J 1ED8 1ED9 1ELX 1ELY 1ELZ 1EW8 1EW9 1HJK 1HQA 1KH4 1KH5 1KH7 1KH9 1KHJ 1KHK 1KHL 1KHN 1URA 1URB 1Y6V 1Y7A 2ANH 2G9Y 2GA3 2MLY 3BDF 3BDG 3BDH 3CMR 3DPC 3DYC 3TG0 4KM4 4YR1 1W26 2MLX 2MLZ 2VRH
DBJ BAB33856 BAE76164 BAG75928 BAI23756 BAI29227 BAB33913 BAD98926 BAE76216 BAG75986 BAI23810
EMBL CAA28257 CAP74918 CAQ30851 CAQ97255 CAR01727 CAP74970 CAQ30908 CAQ97312 CAR01780 CAR06670
GB AAA24359 AAA24363 AAA24364 AAA24365 AAA24366 AAA62791 AAB40192 AAC73539 AAG54786 AAN42037
REF NP_308460 NP_414917 NP_706185 WP_000089619 WP_000089625 NP_286178 NP_308517 NP_414970 NP_706330 NP_752485
SP P00634 A1A8A5 A7ZIJ4 A7ZX94 B1J012 B1LJJ3
BMRB 19835 19837
AlphaFold P00634 A1A8A5 A7ZIJ4 A7ZX94 B1J012 B1LJJ3

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