BMRB Entry 19938

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PDB ID: 6ali
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19938
MolProbity Validation Chart

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Title:
Solution NMR structure of a putative thioredoxin (ECH_0218) in the oxidized state from Ehrlichia chaffeensis, the etiological agent responsible for human monocytic ehrlichiosis. Seattle Structural Genomics Center for Infectious Disease target EhchA.00546.a
Deposition date:
2014-04-24
Original release date:
2014-05-12
Authors:
Buchko, Garry
Citation:

Citation: Buchko, Garry; Hewitt, Stephan; Van Voorhis, Wesley; Myler, Peter. "Solution structure of the Ehrlichia chaffeensis thioredoxin ECH_0218 in the reduced and oxidized states: disorder around the CXXC active site"  .

Assembly members:

Assembly members:
entity, polymer, 256 residues, 28706.621 Da.

Natural source:

Natural source:   Common Name: a-proteobacteria   Taxonomy ID: 205920   Superkingdom: Bacteria   Kingdom: not available   Genus/species: EHRLICHIA CHAFFEENSIS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: AVA0421

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MAHHHHHHMGTLEAQTQGPG SMIEQIGDSEFDNKVTSCND NILILVDFWAPWCGPCRSLE PQLEKLAQQYTENVKIYKIN IEDNQDVATQYGVSAIPTIL MFKNGKKLSQVIGADISKII SEINNNINMAHHHHHHMGTL EAQTQGPGSMIEQIGDSEFD NKVTSCNDNILILVDFWAPW CGPCRSLEPQLEKLAQQYTE NVKIYKINIEDNQDVATQYG VSAIPTILMFKNGKKLSQVI GADISKIISEINNNIN

Data sets:
Data typeCount
13C chemical shifts352
15N chemical shifts117
1H chemical shifts700

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1putative thioredoxin (ECH_0218) in the oxidized state1

Entities:

Entity 1, putative thioredoxin (ECH_0218) in the oxidized state 256 residues - 28706.621 Da.

1   METALAHISHISHISHISHISHISMETGLY
2   THRLEUGLUALAGLNTHRGLNGLYPROGLY
3   SERMETILEGLUGLNILEGLYASPSERGLU
4   PHEASPASNLYSVALTHRSERCYSASNASP
5   ASNILELEUILELEUVALASPPHETRPALA
6   PROTRPCYSGLYPROCYSARGSERLEUGLU
7   PROGLNLEUGLULYSLEUALAGLNGLNTYR
8   THRGLUASNVALLYSILETYRLYSILEASN
9   ILEGLUASPASNGLNASPVALALATHRGLN
10   TYRGLYVALSERALAILEPROTHRILELEU
11   METPHELYSASNGLYLYSLYSLEUSERGLN
12   VALILEGLYALAASPILESERLYSILEILE
13   SERGLUILEASNASNASNILEASNMETALA
14   HISHISHISHISHISHISMETGLYTHRLEU
15   GLUALAGLNTHRGLNGLYPROGLYSERMET
16   ILEGLUGLNILEGLYASPSERGLUPHEASP
17   ASNLYSVALTHRSERCYSASNASPASNILE
18   LEUILELEUVALASPPHETRPALAPROTRP
19   CYSGLYPROCYSARGSERLEUGLUPROGLN
20   LEUGLULYSLEUALAGLNGLNTYRTHRGLU
21   ASNVALLYSILETYRLYSILEASNILEGLU
22   ASPASNGLNASPVALALATHRGLNTYRGLY
23   VALSERALAILEPROTHRILELEUMETPHE
24   LYSASNGLYLYSLYSLEUSERGLNVALILE
25   GLYALAASPILESERLYSILEILESERGLU
26   ILEASNASNASNILEASN

Related Database Links:

PDB 2MCS 2MOD

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