BMRB Entry 19957

Click here to enlarge.

PDB ID: 2nbj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR19957
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Assignment of DNA-MC1 protein complex
Deposition date:
2014-05-07
Original release date:
2014-10-14
Authors:
Loth, Karine; Landon, Celine; Paquet, Francoise
Citation:

Citation: Loth, Karine; Landon, Celine; Paquet, Francoise. "Chemical shifts assignments of the archaeal MC1 protein and a strongly bent 15 base pairs DNA duplex in complex"  Biomol. NMR Assignments ., .-. (2014).
PubMed: 25212183

Assembly members:

Assembly members:
MC1, polymer, 93 residues, Formula weight is not available
DNA1, polymer, 15 residues, Formula weight is not available
DNA2, polymer, 15 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. Coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET24a-mc1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MC1: SNTRNFVLRDEDGNEHGVFT GKQPRQAALKAANRGSGTKA NPDIIRLRERGTKKVHVFKA WKEIVDAPKNRPAWMPEKIS KPFVKKERIEKLE
DNA1: AAAAACACACACCCA
DNA2: TGGGTGTGTGTTTTT

Data typeCount
13C chemical shifts78
15N chemical shifts103
1H chemical shifts873

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MC11
2DNA12
3DNA23

Entities:

Entity 1, MC1 93 residues - Formula weight is not available

1   SERASNTHRARGASNPHEVALLEUARGASP
2   GLUASPGLYASNGLUHISGLYVALPHETHR
3   GLYLYSGLNPROARGGLNALAALALEULYS
4   ALAALAASNARGGLYSERGLYTHRLYSALA
5   ASNPROASPILEILEARGLEUARGGLUARG
6   GLYTHRLYSLYSVALHISVALPHELYSALA
7   TRPLYSGLUILEVALASPALAPROLYSASN
8   ARGPROALATRPMETPROGLULYSILESER
9   LYSPROPHEVALLYSLYSGLUARGILEGLU
10   LYSLEUGLU

Entity 2, DNA1 15 residues - Formula weight is not available

1   DADADADADADCDADCDADC
2   DADCDCDCDA

Entity 3, DNA2 15 residues - Formula weight is not available

1   DTDGDGDGDTDGDTDGDTDG
2   DTDTDTDTDT

Related Database Links:

PDB 1T23 2KHL
GB AKB13417 AKB15948 ALK04717
REF WP_048167444
SP P12770
AlphaFold P12770

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks