BMRB Entry 20076
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR20076
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Title: NMR Solution Structure of a TC5b_D9E miniprotein PubMed: 18161949
Deposition date: 2009-02-13 Original release date: 2009-03-12
Authors: Hudaky, Peter; Straner, Pal; Rovo, Petra
Citation: Hudaky, Peter; Straner, Pal; Farkas, Viktor; Varadi, Gyorgyi; Toth, Gabor; Perczel, Andras. "Cooperation between a Salt Bridge and the Hydrophobic Core Triggers Fold Stabilization in a Trp-Cage Miniprotein" Biochemistry 47, 1007-1016 (2008).
Assembly members:
TC6b, polymer, 20 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
TC6b: NLYIQWLKEGGPSSGRPPPS
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 134 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | TC6b | 1 |
Entities:
Entity 1, TC6b 20 residues - Formula weight is not available
| 1 | ASN | LEU | TYR | ILE | GLN | TRP | LEU | LYS | GLU | GLY | |
| 2 | GLY | PRO | SER | SER | GLY | ARG | PRO | PRO | PRO | SER |
Samples:
sample_1: TC6b 2 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 6.61; temperature: 282 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - geometry optimization, structure solution
SPARKY, Goddard - chemical shift assignment, peak picking
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker DRX 500 MHz
Related Database Links:
| PDB |