BMRB Entry 25002

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PDB ID: 2mpw
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25002
MolProbity Validation Chart

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Title:
Solution structure of the LysM region of the E. coli Intimin periplasmic domain
Deposition date:
2014-06-05
Original release date:
2014-11-10
Authors:
Coles, Murray; Chaubey, Manish; Leo, Jack; Linke, Dirk; Schuetz, Monika; Goetz, Friedrich; Autenrieth, Ingo
Citation:

Citation: Leo, Jack; Oberhettinger, Philipp; Chaubey, Manish; Schuetz, Monika; Kuehner, Daniel; Goetz, Friedrich; Coles, Murray; Autenrieth, Ingo; Linke, Dirk. "The intimin periplasmic domain mediates dimerisation and binding to peptidoglycan"  Mol. Microbiol. ., .-. (2014).
PubMed: 25353290

Assembly members:

Assembly members:
LysM, polymer, 115 residues, 12568.278 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pASK-IBA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LysM: MNGENYFKLGSDSKLLTHNS YQNRLFYTLKTGETVADLSK SQDINLSTIWSLNKHLYSSE SEMMKAAPGQQIILPLKKLP FEYSALPLLGSAPLVAAGGV AGHTNGSGSENLYFQ

Data sets:
Data typeCount
1H chemical shifts550
13C chemical shifts315
15N chemical shifts82

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1LysM1

Entities:

Entity 1, LysM 115 residues - 12568.278 Da.

A39 is mutated to M for cloning purposes

1   METASNGLYGLUASNTYRPHELYSLEUGLY
2   SERASPSERLYSLEULEUTHRHISASNSER
3   TYRGLNASNARGLEUPHETYRTHRLEULYS
4   THRGLYGLUTHRVALALAASPLEUSERLYS
5   SERGLNASPILEASNLEUSERTHRILETRP
6   SERLEUASNLYSHISLEUTYRSERSERGLU
7   SERGLUMETMETLYSALAALAPROGLYGLN
8   GLNILEILELEUPROLEULYSLYSLEUPRO
9   PHEGLUTYRSERALALEUPROLEULEUGLY
10   SERALAPROLEUVALALAALAGLYGLYVAL
11   ALAGLYHISTHRASNGLYSERGLYSERGLU
12   ASNLEUTYRPHEGLN

Related Database Links:

GB BAL46976 AAA62775 AAB52913 AAC31504 AAC38392 AAD05498
PDB 2MPW
DBJ BAB37982 BAD20939 BAD20940 BAD20941 BAD20942
EMBL CAA42967 CAA77642 CAC21552 CAC24714 CAC59747
PRF 1905283A
REF NP_312586 WP_000430959 WP_000627883 WP_000627884 WP_000627885
SP P19809 P43261
AlphaFold P19809 P43261

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