BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25129

Title: cBru9a   PubMed: 26172377

Deposition date: 2014-08-05 Original release date: 2015-07-20

Authors: Akcan, Muharrem; Clark, Richard; Daly, Norelle; Conibear, Anne; de Faoite, Andrew; Heghinian, Mari; Adams, David; Mari, Frank; Craik, David

Citation: Akcan, Muharrem; Clark, Richard; Daly, Norelle; Conibear, Anne; de Faoite, Andrew; Heghinian, Mari; Adams, David; Mari, Frank; Craik, David. "Transforming conotoxins into cyclotides: backbone cyclization of P-superfamily conotoxins"  Biopolymers ., .-. (2015).

Assembly members:
cBru9a, polymer, 27 residues, 2809.185 Da.

Natural source:   Common Name: Wood's brown cone   Taxonomy ID: 101289   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Conus brunneus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
cBru9a: SCGGSCFGGCWXGCSCYART CFRDGLP

Data sets:
Data typeCount
1H chemical shifts137

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cBru9a1

Entities:

Entity 1, cBru9a 27 residues - 2809.185 Da.

1   SERCYSGLYGLYSERCYSPHEGLYGLYCYS
2   TRPHYPGLYCYSSERCYSTYRALAARGTHR
3   CYSPHEARGASPGLYLEUPRO

Samples:

sample_1: cBru9a0.05 – 0.2 mM; H2O 90%; D2O 10%

sample_conditions_1: pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker Avance 900 MHz

Related Database Links:

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