BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 25146

Title: Solid-state NMR chemical shifts of amyloid-like fibrils formed by huntingtin N-terminal fragments (httNTQ30P10K2)   PubMed: 25280367

Deposition date: 2014-08-12 Original release date: 2014-10-14

Authors: Hoop, Cody; Kar, Karunakar; Wetzel, Ronald; van der Wel, Patrick

Citation: Hoop, Cody; Lin, Hsiang-Kai; Kar, Karunakar; Hou, Zhipeng; Poirier, Michelle; Wetzel, Ronald; van der Wel, Patrick. "Polyglutamine amyloid core boundaries and flanking domain dynamics in huntingtin fragment fibrils determined by solid-state NMR"  Biochemistry 53, 6653-6666 (2014).

Assembly members:
httNTQ30P10K2, polymer, 59 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
httNTQ30P10K2: MATLEKLMKAFESLKSFQQQ QQQQQQQQQQQQQQQQQQQQ QQQQQQQPPPPPPPPPPKK

Data typeCount
13C chemical shifts138
15N chemical shifts12

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1httNTQ30P10K2, Chain A1
2httNTQ30P10K2, Chain B1

Entities:

Entity 1, httNTQ30P10K2, Chain A 59 residues - Formula weight is not available

1   METALATHRLEUGLULYSLEUMETLYSALA
2   PHEGLUSERLEULYSSERPHEGLNGLNGLN
3   GLNGLNGLNGLNGLNGLNGLNGLNGLNGLN
4   GLNGLNGLNGLNGLNGLNGLNGLNGLNGLN
5   GLNGLNGLNGLNGLNGLNGLNPROPROPRO
6   PROPROPROPROPROPROPROLYSLYS

Samples:

LQP-labeled: httNTQ30P10K2, [U-13C; U-15N]- L7, Q47, P48, 8 mg

LAQ-labeled: httNTQ30P10K2, [U-13C; U-15N]- L4, A10, Q46, 8 mg

LKSQ-labeled: httNTQ30P10K2, [U-13C; U-15N]- L4, K6, S16, Q19, 1.8 mg

MA-labeled: httNTQ30P10K2, [U-13C; U-15N]- M8, A10, 8 mg

ALF-labeled: httNTQ30P10K2, [U-13C; U-15N]- A2, L7, F17, 2 mg

AFLQ-labeled: httNTQ30P10K2, [U-13C; U-15N]- A10, F11, L14, Q18, 3 mg

275_K: temperature: 275 K

Experiments:

NameSampleSample stateSample conditions
2D 13C-13C DARRLQP-labeledsolid275_K
2D 13C-13C DARRLAQ-labeledsolid275_K
2D 13C-13C DARRLKSQ-labeledsolid275_K
2D 13C-13C DARRMA-labeledsolid275_K
2D 15N-13C TEDORLQP-labeledsolid275_K
2D 13C-13C DARRALF-labeledsolid275_K
2D 13C-13C DARRAFLQ-labeledsolid275_K

Software:

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz