BMRB Entry 25188

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PDB ID: 2mtv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25188
MolProbity Validation Chart

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Title:
Solution Structure of the YTH Domain of YT521-B in complex with N6-Methyladenosine containing RNA
Deposition date:
2014-09-01
Original release date:
2014-11-17
Authors:
Theler, Dominik; Dominguez, Cyril; Blatter, Markus; Boudet, Julien; Allain, Frederic
Citation:

Citation: Theler, Dominik; Dominguez, Cyril; Blatter, Markus; Boudet, Julien; Allain, Frederic. "Solution structure of the YTH domain in complex with N6-methyladenosine RNA: a reader of methylated RNA"  Nucleic Acids Res. 42, 13911-13919 (2014).
PubMed: 25389274

Assembly members:

Assembly members:
entity_1, polymer, 156 residues, 17508.334 Da.
RNA_(5'-R(*UP*GP*(6MA)P*CP*AP*C)-3'), polymer, 6 residues, 1873.230 Da.

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTYB11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: QTSKLKSVLQDARFFLIKSN NHENVSLAKAKGVWSTLPVN EKKLNLAFRSARSVILIFSV RESGKFQGFARLSSESHHGG SPIHWVLPAGMSAKMLGGVF KIDWICRRELPFTKSAHLTN PWNEHKPVKIGRDGQEIELE CGTQLCLLFPPDESID
RNA_(5'-R(*UP*GP*(6MA)P*CP*AP*C)-3'): UGXCAC

Data sets:
Data typeCount
13C chemical shifts683
15N chemical shifts155
1H chemical shifts1138

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2RNA (5'-R(*UP*GP*(6MA)P*CP*AP*C)-3')2

Entities:

Entity 1, entity_1 156 residues - 17508.334 Da.

1   GLNTHRSERLYSLEULYSSERVALLEUGLN
2   ASPALAARGPHEPHELEUILELYSSERASN
3   ASNHISGLUASNVALSERLEUALALYSALA
4   LYSGLYVALTRPSERTHRLEUPROVALASN
5   GLULYSLYSLEUASNLEUALAPHEARGSER
6   ALAARGSERVALILELEUILEPHESERVAL
7   ARGGLUSERGLYLYSPHEGLNGLYPHEALA
8   ARGLEUSERSERGLUSERHISHISGLYGLY
9   SERPROILEHISTRPVALLEUPROALAGLY
10   METSERALALYSMETLEUGLYGLYVALPHE
11   LYSILEASPTRPILECYSARGARGGLULEU
12   PROPHETHRLYSSERALAHISLEUTHRASN
13   PROTRPASNGLUHISLYSPROVALLYSILE
14   GLYARGASPGLYGLNGLUILEGLULEUGLU
15   CYSGLYTHRGLNLEUCYSLEULEUPHEPRO
16   PROASPGLUSERILEASP

Entity 2, RNA (5'-R(*UP*GP*(6MA)P*CP*AP*C)-3') 6 residues - 1873.230 Da.

1   UG6MZCAC

Related Database Links:

PDB 2MTV 2YUD 4R3H 4R3I
DBJ BAA23885 BAB71181 BAB85552 BAD32590 BAG10510
EMBL CAH91339
GB AAD55973 AAH22697 AAH41119 AAH53863 AAI02815
REF NP_001026902 NP_001039985 NP_001125792 NP_588611 NP_596914
SP E9Q5K9 Q96MU7 Q9QY02
TPG DAA28607
AlphaFold E9Q5K9 Q96MU7 Q9QY02

Download HSQC peak lists in one of the following formats:
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